Western Blot (WB)
(Sample Data: Western blot analysis of ALK2 in A549 cell lysate with ALK2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)
Rabbit anti-Human, Mouse ALK2 Polyclonal Antibody | anti-ALK2 antibody
Anti-ALK2
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Affinity chromatography
Synonyms
ALK2; Polyclonal Antibody; Anti-ALK2; anti-ALK2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the ALK2 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-ALK2 antibody
Western Blot (WB), ELISA
Immunogen
ALK2 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of the human ALK2
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of ALK2 in A549 cell lysate with ALK2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Sample Data: Western blot analysis of ALK2 in A549 cell lysate with ALK2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)
Related Product Information for anti-ALK2 antibody
ALK2 is a receptor serine/threonine kinase that is member of the ALK family and is upstream of signaling pathway involving the SMAD proteins especially SMAD1/5/8. Knockdown of ALK2, but not TGFbetaRI (ALK5), abrogates endoglin-mediated decrease in cell motility of prostate cancer cells and constitutively active ALK2 is sufficient to restore a low-motility phenotype in endoglin deficient cells (1). Therefore, endoglin decreases prostate cancer cell motility through activation of the ALK2-Smad1 pathway. ALK2 is the key gene involved in Fibrodysplasia ossificans progressiva (FOP), a rare autosomal dominant congenital disorder characterized by progressive heterotopic bone formation in muscle tissues (2).
Product Categories/Family for anti-ALK2 antibody
References
1. Craft, C.S. et al: Endoglin inhibits prostate cancer motility via activation of the ALK2-Smad1 pathway. Oncogene. 2007 Nov 8;26(51):7240-50.
2. Shore, E. M. et al: A recurrent mutation in the BMP type I receptor ACVR1 causes inherited and sporadic fibrodysplasia ossificans progressiva. Nature Genet. 38: 525-527, 2006
2. Shore, E. M. et al: A recurrent mutation in the BMP type I receptor ACVR1 causes inherited and sporadic fibrodysplasia ossificans progressiva. Nature Genet. 38: 525-527, 2006
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Product Notes
The ALK2 (Catalog #AAA515810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ALK2 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ALK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA. Researchers should empirically determine the suitability of the ALK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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