Rabbit anti-Mouse Alk Polyclonal Antibody | anti-Alk antibody

Mouse Alk Antibody (P1517)

Cat. Num. AAA9207330

• Gene Names: Alk; Tcrz; CD246
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Alk; Polyclonal Antibody; Mouse Alk Antibody (P1517); ALK tyrosine kinase receptor; Anaplastic lymphoma kinase; CD246; anti-Alk antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Alk antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1517-1550 amino acids Mouse Alk.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.41 (varies by lot)
• Sequence Length: 1,621

• Applicable Applications for anti-Alk antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-Alk Antibody (P1517)at 1:2000 dilution + mouse cerebellum lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 175 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-Alk antibody

Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.

References

Iwahara T.,et al.Oncogene 14:439-449(1997).
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Motegi A.,et al.J. Cell Sci. 117:3319-3329(2004).
Vernersson E.,et al.Gene Expr. Patterns 6:448-461(2006).
Kuo A.H.,et al.Oncogene 26:859-869(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 110347475
• NCBI GeneID: 11682
• NCBI Accession #: NP_031465.2
• NCBI GenBank Nucleotide #: NM_007439.2
• UniProt Accession #: P97793; E9QKV3
• Molecular Weight: 174948
• NCBI Official Full Name: ALK tyrosine kinase receptor
• NCBI Official Synonym Full Names: anaplastic lymphoma kinase
• NCBI Official Symbol: Alk
• NCBI Official Synonym Symbols: Tcrz; CD246
• NCBI Protein Information: ALK tyrosine kinase receptor
• UniProt Protein Name: ALK tyrosine kinase receptor
• Protein Family: Alkylsulfatase
• UniProt Gene Name: Alk
• UniProt Entry Name: ALK_MOUSE

Uniprot Description

ALK: a tyrosine kinase of the ALK family. Plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. Translocated and expressed as a fusion protein in anaplastic lymphoma. About one third of large-cell lymphomas are caused by a t(2;5)(p23;q35) translocation that fuses ALK to nucleophosmin (NPM1A). Other cases caused by fusions of ALK to moesin, non-muscle myosin heavy chain 9, clathrin heavy chain and other genes. Several fusions also seen in inflammatory myofibroblastic tumors, and expression has been briefly noted in a range of tumors

Protein type: EC 2.7.10.1; Protein kinase, TK; Oncoprotein; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; Alk family

Cellular Component: protein complex; membrane; axon; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: nervous system development; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; neuron development; signal transduction; phosphorylation; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway