Western Blot (WB)
((0.3ug/ml) staining of Human (A) and Mouse (B) Liver, and Rat (C) Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Goat ALDH1A1 Polyclonal Antibody | anti-ALDH1A1 antibody
Goat anti-ALDH1A1 (C Terminus) Antibody
Immunohistochemistry: This antibody has been successfully used in IHC on mouse Peyer's patches as described in the following paper: Iwata et al, Immunity.2004 Oct;21(4): 527-38.; PMID: 15485630.
Western Blot: Approx 55kDa band observed in Human and Mouse Liver, and Rat Testis lysates (calculated MW of 54.5kDa according to Human NP_000680.2; Mouse NP_038495.2 and Rat NP_071852.2). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human (A) and Mouse (B) Liver, and Rat (C) Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
((0.3ug/ml) staining of Human (A) and Mouse (B) Liver, and Rat (C) Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
(HEK293 overexpressing ALDH1A1 (RC200723) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing ALDH1A1 (RC200723) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]
Uniprot Description
ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.
Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - retinol; Apoptosis; EC 1.2.1.36; GAPs; GAPs, Ras
Chromosomal Location of Human Ortholog: 9q21.13
Cellular Component: cytoplasm; cytosol
Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; benzaldehyde dehydrogenase (NAD+) activity; GTPase activator activity; retinal dehydrogenase activity
Biological Process: aldehyde metabolic process; ethanol oxidation; positive regulation of GTPase activity; retinol metabolic process
Research Articles on ALDH1A1
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Product Notes
The ALDH1A1 aldh1a1 (Catalog #AAA420981) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ALDH1A1 (C Terminus) Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH1A1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: This antibody has been successfully used in IHC on mouse Peyer's patches as described in the following paper: Iwata et al, Immunity.2004 Oct;21(4): 527-38.; PMID: 15485630. Western Blot: Approx 55kDa band observed in Human and Mouse Liver, and Rat Testis lysates (calculated MW of 54.5kDa according to Human NP_000680.2; Mouse NP_038495.2 and Rat NP_071852.2). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the ALDH1A1 aldh1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EVKTVTVKIS QKNS. It is sometimes possible for the material contained within the vial of "ALDH1A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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