Western Blot (WB)
(Western Blot: Sample: Rat Liver Tissue.)
Rabbit anti-Human Aldehyde Dehydrogenase 1 Family Polyclonal Antibody | anti-ALDH1A1 antibody
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Rat Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Rat Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ALDH1A1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant ALDH1A1, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Cancer Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Cancer Tissue)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]
Uniprot Description
ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.
Protein type: Apoptosis; Cofactor and Vitamin Metabolism - retinol; EC 1.2.1.36; GAP; GAP, Ras; Oxidoreductase
Chromosomal Location of Human Ortholog: 9q21.13
Cellular Component: cytoplasm; cytosol; extracellular exosome
Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; benzaldehyde dehydrogenase (NAD+) activity; GTPase activator activity; NAD binding; retinal dehydrogenase activity
Biological Process: aldehyde metabolic process; ethanol oxidation; positive regulation of GTPase activity; retinoid metabolic process; retinol metabolic process
Research Articles on ALDH1A1
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Product Notes
The ALDH1A1 aldh1a1 (Catalog #AAA2032270) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aldehyde Dehydrogenase 1 Family can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ALDH1A1 aldh1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aldehyde Dehydrogenase 1 Family, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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