Goat albumin Polyclonal Antibody | anti-ALB antibody

Goat anti Human albumin, conjugated with Biotin

Cat. Num. AAA573594

• Gene Names: ALB; PRO0883; PRO0903; PRO1341
• Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentrations of
• Applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Dot Blot, Immunoblot, Western Blot
• Purity: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG (7S) fraction by DEAE-chromatography.
• Synonyms: albumin; Polyclonal Antibody; Goat anti Human albumin; conjugated with Biotin; anti-ALB antibody

• Host: Goat
• Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentrations of
• Clonality: Polyclonal
• Specificity: The defined antibody specificity is directed to albumin as tested against human sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against human serum and the purified albumin.
• Purity/Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG (7S) fraction by DEAE-chromatography.
• Form/Format: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

• Applicable Applications for anti-ALB antibody: ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
• Conjugation: Biotin
• Immunogen: Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiologica! molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
• Physicochemical characteristics: IgG protein concentration 7.5 mg/ml. Biotin/lgG protein molar ratio (B/P) approximately 5.7. No foreign proteins added.
• Marker: N-Hydroxysuccinimidobiotin
• Handling: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
• Caution: This immunoconjugate should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro laboratory research purposes only.
• Preparation and Storage: Lyophilized at +4 Degree C = at least 10 years; reconstituted at or below -20 Degree C = 3-5 years; reconstituted at +4 Degree C = 7 days

Related Product Information for anti-ALB antibody

In immunocytochemical and immunohistochemical use for the detection of albumin at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; in non-isotopic assay methodology (e.g. ELISA) to measure albumin in human serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used.

This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Working dilutions for histochemical and cytochemical use are usually between 1: 1 00 and 1: 500; in ELISA and comparable non-precipitating antibody-binding assays between 1 :5,000 and 1 :50,000.

Product Categories/Family for anti-ALB antibody

Conjugated Secondary Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 178344
• NCBI GeneID: 213
• UniProt Accession #: P02768; O95574; P04277; Q13140; Q645G4; Q68DN5; Q6UXK4; Q86YG0; Q9P157; Q9P1I7; Q9UHS3; Q9UJZ0
• Molecular Weight: 69,367 Da
• NCBI Official Full Name: albumin
• NCBI Official Synonym Full Names: albumin
• NCBI Official Symbol: ALB
• NCBI Official Synonym Symbols: PRO0883; PRO0903; PRO1341
• NCBI Protein Information: serum albumin; albumin (32 AA); albumin (AA 34); growth-inhibiting protein 20; cell growth inhibiting protein 42
• UniProt Protein Name: Serum albumin
• Protein Family: Albumin
• UniProt Gene Name: ALB
• UniProt Entry Name: ALBU_HUMAN

NCBI Description

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]

Uniprot Description

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Carrier

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus

Molecular Function: antioxidant activity; toxin binding; protein binding; enzyme binding; copper ion binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding

Biological Process: platelet activation; receptor-mediated endocytosis; sodium-independent organic anion transport; maintenance of mitochondrion localization; bile acid metabolic process; hemolysis by symbiont of host red blood cells; lipoprotein metabolic process; cellular response to starvation; bile acid and bile salt transport; response to organic substance; response to mercury ion; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; transmembrane transport; positive regulation of circadian sleep/wake cycle, non-REM sleep; response to nutrient; negative regulation of apoptosis

Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic