Rabbit anti-Human, Mouse ALB Polyclonal Antibody | anti-ALB antibody

ALB, CT (Serum Albumin, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, DKFZp779N1935, PRO0883, PRO1341) (MaxLight 405)

Cat. Num. AAA6462198

• Gene Names: ALB; HSA; PRO0883; PRO0903; PRO1341
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ALB; Polyclonal Antibody; CT (Serum Albumin; GIG20; GIG42; PRO0903; PRO1708; PRO2044; PRO2619; PRO2675; UNQ696/PRO1341; DKFZp779N1935; PRO0883; PRO1341) (MaxLight 405); anti-ALB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALB. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-ALB antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ALB (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ALB antibody (C-term) in mouse liver tissue lysates (35ug/lane). ALB (arrow) was detected using the purified Pab.)

Western Blot (WB)

(ALB Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ALB antibody detected the ALB protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ALB Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(ALB Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ALB antibody

Antibodies; Albumin

References

1. Liu, Z., Biophys. J. 96(10), 3917-3925 (2009). 2. Minchiotti, L., Hum. Mutat. 29(8), 1007-1016 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4502027
• NCBI GeneID: 213
• NCBI Accession #: NP_000468
• NCBI GenBank Nucleotide #: NM_000477.6
• UniProt Accession #: P02768; O95574; P04277; Q13140; Q645G4; Q68DN5; Q6UXK4; Q86YG0; Q8IUK7; Q9P157; Q9P1I7; E7ESS9
• Molecular Weight: 68kDa (597aa) 50-70kDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: serum albumin preproprotein
• NCBI Official Synonym Full Names: albumin
• NCBI Official Symbol: ALB
• NCBI Official Synonym Symbols: HSA; PRO0883; PRO0903; PRO1341
• NCBI Protein Information: serum albumin
• UniProt Protein Name: Serum albumin
• Protein Family: Albumin
• UniProt Gene Name: ALB
• UniProt Entry Name: ALBU_HUMAN

NCBI Description

This gene encodes the most abundant protein in human blood. This protein functions in the regulation of blood plasma colloid osmotic pressure and acts as a carrier protein for a wide range of endogenous molecules including hormones, fatty acids, and metabolites, as well as exogenous drugs. Additionally, this protein exhibits an esterase-like activity with broad substrate specificity. The encoded preproprotein is proteolytically processed to generate the mature protein. A peptide derived from this protein, EPI-X4, is an endogenous inhibitor of the CXCR4 chemokine receptor. [provided by RefSeq, Jul 2016]

Uniprot Description

albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Carrier; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus

Molecular Function: antioxidant activity; toxin binding; protein binding; copper ion binding; enzyme binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding

Biological Process: platelet activation; receptor-mediated endocytosis; sodium-independent organic anion transport; bile acid metabolic process; maintenance of mitochondrion localization; lipoprotein metabolic process; hemolysis by symbiont of host red blood cells; cellular response to starvation; response to organic substance; response to mercury ion; bile acid and bile salt transport; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; transmembrane transport; positive regulation of circadian sleep/wake cycle, non-REM sleep; response to nutrient; negative regulation of apoptosis

Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic