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Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse AGXT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Alanine Glyoxylate Aminotransferase 2 Polyclonal Antibody | anti-AGXT2 antibody
Polyclonal Antibody to Alanine Glyoxylate Aminotransferase 2 (AGXT2)
Gene Names
Agxt2; AI303810; AI663818
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Alanine Glyoxylate Aminotransferase 2; Polyclonal Antibody; Polyclonal Antibody to Alanine Glyoxylate Aminotransferase 2 (AGXT2); AGT2; Beta-Alanine-Pyruvate Aminotransferase; (R)-3-amino-2-methylpropionate--pyruvate transaminase; anti-AGXT2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against AGXT2. It has been selected for its ability to recognize AGXT2 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
514
Applicable Applications for anti-AGXT2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Fragment
AGXT2 (Met226~Lys513)
Conjugate
No Conjugate
Cross Reactivity
Mouse
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant AGXT2 (Met226~Lys513) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2073268
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse AGXT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse AGXT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,115 Da
NCBI Official Full Name
alanine--glyoxylate aminotransferase 2, mitochondrial isoform 1
NCBI Official Synonym Full Names
alanine-glyoxylate aminotransferase 2
NCBI Official Symbol
Agxt2
NCBI Official Synonym Symbols
AI303810; AI663818
NCBI Protein Information
alanine--glyoxylate aminotransferase 2, mitochondrial
UniProt Protein Name
Alanine--glyoxylate aminotransferase 2, mitochondrial
Protein Family
UniProt Gene Name
Agxt2
UniProt Synonym Gene Names
AGT 2
Uniprot Description
AGXT2: is a class III pyridoxal-phosphate-dependent mitochondrial aminotransferase. It catalyzes the conversion of glyoxylate to glycine using L-alanine as the amino donor. [provided by RefSeq, Dec 2008]
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 2.6.1.40; EC 2.6.1.44; Mitochondrial; Transferase
Chromosomal Location of Human Ortholog: 15|15 A1
Cellular Component: mitochondrion
Molecular Function: (R)-3-amino-2-methylpropionate-pyruvate transaminase activity; alanine-glyoxylate transaminase activity; beta-alanine-pyruvate transaminase activity; catalytic activity; identical protein binding; pyridoxal phosphate binding; transaminase activity; transferase activity
Biological Process: glycine biosynthetic process, by transamination of glyoxylate; glyoxylate catabolic process; L-alanine catabolic process, by transamination; positive regulation of nitric oxide biosynthetic process
Research Articles on AGXT2
Similar Products
Product Notes
The AGXT2 agxt2 (Catalog #AAA2033872) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Alanine Glyoxylate Aminotransferase 2 (AGXT2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Alanine Glyoxylate Aminotransferase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the AGXT2 agxt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alanine Glyoxylate Aminotransferase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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