Rabbit AKT2 Polyclonal Antibody | anti-AKT2 antibody

AKT2 (Phospho-S474) Polyclonal Antibody

Cat. Num. AAA3005221

• Gene Names: AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AKT2; Polyclonal Antibody; AKT2 (Phospho-S474) Polyclonal Antibody; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; PKBbeta; anti-AKT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-AKT2 (S474) polyclonal antibody detects endogenous levels of AKT2 protein only when phosphorylated at Ser474.This antibody does not cross-react with AKT1 and AKT3 protein when phosphorylated at the corresponding residues.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 481

• Applicable Applications for anti-AKT2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human AKT2 around the phosphorylation site of Serine 474.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-AKT2 (S474) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with EGF (100ng/ml,15 minutes) whole cell lysate (40ug) Lane3:HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug) Lane4:The Brain tissue lysate of Rat (40ug) Lane5:The Brain tissue lysate of Mouse (40ug) Lane6:C6 whole cell lysate (40ug) Lane7:AML-12 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-AKT2 (S474) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-AKT2 antibody

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 4502023
• NCBI GeneID: 208
• NCBI Accession #: NP_001617.1
• NCBI GenBank Nucleotide #: NP_001617.1
• UniProt Accession #: P31751
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: RAC-beta serine/threonine-protein kinase isoform 1
• NCBI Official Synonym Full Names: AKT serine/threonine kinase 2
• NCBI Official Symbol: AKT2
• NCBI Official Synonym Symbols: PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
• NCBI Protein Information: RAC-beta serine/threonine-protein kinase
• UniProt Protein Name: RAC-beta serine/threonine-protein kinase
• Protein Family: Potassium channel
• UniProt Gene Name: AKT2
• UniProt Synonym Gene Names: PKB beta
• UniProt Entry Name: AKT2_HUMAN

NCBI Description

This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling. [provided by RefSeq, Nov 2019]

Uniprot Description

Akt2: an AGC kinase. Plays critical roles in glucose metabolism and the development or maintenance of proper adipose tissue and islet mass for which other Akt/PKB isoforms are unable to fully compensate. Amplified and overexpressed in human ovarian carcinoma cell lines and amplified in some primary ovarian and pancreatic tumors. Antisense blocks invasiveness in xenografts. Expressed in several insulin-responsive tissues, and one case of Type II diabetes has been associated with a likely LOF point mutation. Mouse mutants have defects in insulin response.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Oncoprotein; AGC group; AKT family

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: nucleoplasm; plasma membrane; cell cortex; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; kinase activity; ATP binding

Biological Process: fat cell differentiation; glycogen biosynthetic process; apoptosis; positive regulation of glycogen biosynthetic process; carbohydrate transport; myelin maintenance in the peripheral nervous system; intracellular protein transport across a membrane; positive regulation of vesicle fusion; protein modification process; glucose metabolic process; signal transduction; regulation of cell migration; protein amino acid phosphorylation; positive regulation of fatty acid beta-oxidation; regulation of translation; positive regulation of glucose import; cellular response to insulin stimulus; insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation

Disease: Hypoinsulinemic Hypoglycemia With Hemihypertrophy; Diabetes Mellitus, Noninsulin-dependent