Rabbit anti-Human Akt2,3 Polyclonal Antibody | anti-KT2/3 antibody

Akt2,3 (AKT-2, Protein Kinase Akt2, Protein Kinase B beta, PKB beta, PKBBeta, PRKBB, Rac Protein Kinase beta, RAC-BETA, RAC-beta Serine/threonine Protein Kinase, RAC-PK-beta, v-AKT Murine Thymoma Viral Oncogene Homolog 2)

Cat. Num. AAA622883

• Gene Names: KT2/3; AKT2; AKT2/3; AKT3; KT2/3; potassium transport 2/3; T10I14.30; T10I14_30
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Akt2; 3; Polyclonal Antibody; 3 (AKT-2; Protein Kinase Akt2; Protein Kinase B beta; PKB beta; PKBBeta; PRKBB; Rac Protein Kinase beta; RAC-BETA; RAC-beta Serine/threonine Protein Kinase; RAC-PK-beta; v-AKT Murine Thymoma Viral Oncogene Homolog 2); Anti -Akt2; anti-KT2/3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Species Crossreactivity: Human
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-KT2/3 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-KT2/3 antibody

Akt, protein kinase B (PKB), is a serine/threonine kinase, which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.

Product Categories/Family for anti-KT2/3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 18415864
• NCBI GeneID: 828311
• NCBI Accession #: NP_567651.1
• NCBI GenBank Nucleotide #: NM_118342.2
• UniProt Accession #: Q38898; Q0WMS8; Q42408; Q9M0L6
• Molecular Weight: 91,308 Da
• NCBI Official Full Name: Potassium channel AKT2/3
• NCBI Official Symbol: KT2/3
• NCBI Official Synonym Symbols: AKT2; AKT2/3; AKT3; KT2/3; potassium transport 2/3; T10I14.30; T10I14_30
• NCBI Protein Information: Potassium channel AKT2/3
• UniProt Protein Name: Potassium channel AKT2/3
• UniProt Gene Name: AKT2
• UniProt Synonym Gene Names: AKT3
• UniProt Entry Name: AKT2_ARATH

NCBI Description

Encodes AKT2, a photosynthate- and light-dependent inward rectifying potassium channel with unique gating properties that are regulated by phosphorylation. Expressed in guard cell protoplasts and in the phloem and xylem of aerial portions of the plant. The channel can coassemble with another K+ channel, KAT1, in vitro. In guard cells, AKT2/3 is responsible for the Ca2+ sensitivity of the K+ uptake channel. In the phloem, it regulates the sucrose/H+ symporters via the phloem potential. AKT2 belongs to the Shaker family K+ channels which include the following groups based on phylogenetic analysis (FEBS Letters (2007) 581: 2357): I (inward rectifying channel): AKT1 (AT2G26650), AKT5 (AT4G32500) and SPIK (also known as AKT6, AT2G25600); II (inward rectifying channel): KAT1 (AT5G46240) and KAT2 (AT4G18290); III (weakly inward rectifying channel): AKT2 (AT4G22200); IV (regulatory subunit involved in inwardly rectifying conductance formation): KAT3 (also known as AtKC1, AT4G32650); V (outward rectifying channel): SKOR (AT3G02850) and GORK (AT5G37500).

Uniprot Description

Function: Highly selective and weak inward-rectifying potassium channel. Plays a role in both loading and unloading potassium into/from the phloem sap. Seems to control sugar loading into phloem via a voltage-dependent process. Blocked by physiological concentrations of external calcium and by external acidification. May interact with the cytoskeleton or with regulatory proteins. Dephosphorylation by PP2CA not only leads to the inhibition of potassium currents but also to an increase of the voltage-dependence of the channel. Ref.11 Ref.14

Subunit structure: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Interacts with the phosphatase PPC2A. May interact with AKT1, KAT1 and KAT3. Ref.7 Ref.9 Ref.12 Ref.14

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: Expressed mainly in the phloem tissues throughout the plant but also, at a lower level, in leaf epiderm, mesophyll and guard cells. Ref.1 Ref.6 Ref.8 Ref.12

Induction: In shoots, strongly induced by abscisic acid (ABA) treatment and reduced after NaCl treatment or potassium starvation. Ref.14

Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Post-translational modification: Dephosphorylated by PP2CA. Ref.12

Sequence similarities: Belongs to the potassium channel family. Plant (TC 1.A.1.4) subfamily. [View classification]Contains 5 ANK repeats.Contains 1 cyclic nucleotide-binding domain.Contains 1 KHA domain.

Sequence caution: The sequence AAA96153.1 differs from that shown. Reason: Erroneous initiation. The sequence AAA96154.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA16770.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB79175.1 differs from that shown. Reason: Erroneous initiation.