Rabbit AKT1S1 Polyclonal Antibody | anti-AKT1S1 antibody

AKT1S1 Antibody

Cat. Num. AAA153593

• Gene Names: AKT1S1; Lobe; PRAS40
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: AKT1S1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: AKT1S1; Polyclonal Antibody; AKT1S1 Antibody; Lobe; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate; AKT1 substrate 1 (proline-rich); anti-AKT1S1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist.
• Purity/Purification: AKT1S1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 276

• Applicable Applications for anti-AKT1S1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1.
• Buffer: AKT1S1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: AKT1S1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of AKT1S1 in rat brain tissue with AKT1S1 antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of AKT1S1 in rat brain tissue with AKT1S1 antibody at 20 μg/ml.)

Related Product Information for anti-AKT1S1 antibody

AKT1S1 Antibody: The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1). AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2). Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3).

NCBI and Uniprot Product Information

• NCBI GI #: 400153980
• NCBI GeneID: 84335
• NCBI Accession #: NP_115751
• NCBI GenBank Nucleotide #: NM_032375.5
• UniProt Accession #: Q96B36; Q96BI4; Q96IK7; Q96NG2; Q9BWR5; A8MTQ1; B2RE93; J3KPM3
• Molecular Weight: Predicted: 30 kDa; ; Observed: 28 kDa
• NCBI Official Full Name: proline-rich AKT1 substrate 1 isoform a
• NCBI Official Synonym Full Names: AKT1 substrate 1 (proline-rich)
• NCBI Official Symbol: AKT1S1
• NCBI Official Synonym Symbols: Lobe; PRAS40
• NCBI Protein Information: proline-rich AKT1 substrate 1; AKT1S1; 40 kDa proline-rich AKT substrate
• UniProt Protein Name: Proline-rich AKT1 substrate 1
• Protein Family: Proline-rich AKT1 substrate
• UniProt Gene Name: AKT1S1
• UniProt Entry Name: AKTS1_HUMAN

NCBI Description

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]

Uniprot Description

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.