Rabbit anti-Human Akt Polyclonal Antibody | anti-Akt1 antibody

Akt, phosphorylated (Thr308) (Rac PKa, PKBa)

Cat. Num. AAA626518

• Gene Names: Akt1; akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Akt; Polyclonal Antibody; phosphorylated (Thr308) (Rac PKa; PKBa); Anti -Akt; anti-Akt1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes phosphorylated human AKT, pT308. Minimal reactivity occurs against non-phosphorylated AKT.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.

• Applicable Applications for anti-Akt1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Dilution: ELISA: 1:20,000-1:70,000 Sandwich ELISA using IgG, H&L GtXRb (HRP) and ABTS as a substrate for 30 minutes at RT.; Western Blot: 1:500
• Immunogen: A synthetic peptide corresponding to aa 302-311 of human AKT protein (KLH). Peptide Sequence: D-G-A-T-M-K-pT-F-C-G.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Akt1 antibody

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: i nhibition of apoptosis and promotion of proliferation.

Product Categories/Family for anti-Akt1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 24647360
• NCBI GeneID: 41957
• NCBI Accession #: NP_732115.1
• NCBI GenBank Nucleotide #: NM_169707.1
• UniProt Accession #: Q8INB9; Q0KI65; Q24293; Q24469; Q24470; Q7JN11; Q8T9A5; Q9VEY7
• Molecular Weight: 68,485 Da
• NCBI Official Full Name: Akt1, isoform B
• NCBI Official Symbol: Akt1
• NCBI Official Synonym Symbols: akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
• NCBI Protein Information: CG4006 gene product from transcript CG4006-RA; Akt kinase; Akt1; Akt1-PA; Akt1-PB; Akt1-PC; CG4006-PA; CG4006-PB; CG4006-PC; dAkt kinase; protein kinase B; related to PKA to PKC protein kinases; related to the A and C kinases
• UniProt Protein Name: RAC serine/threonine-protein kinase
• Protein Family: AKT-interacting protein
• UniProt Gene Name: Akt1
• UniProt Synonym Gene Names: DAkt; DRAC-PK; Dakt1; PKB
• UniProt Entry Name: AKT1_DROME

Uniprot Description

Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Protein kinase, Ser/Thr (non-receptor); Oncoprotein; Protein kinase, AGC; Kinase, protein; EC 2.7.11.1; AGC group; AKT family

Cellular Component: cell surface; cell soma; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; phosphoinositide binding; ATP binding; protein kinase activity

Biological Process: oogenesis; circadian rhythm; positive regulation of cell size; chitin-based embryonic cuticle biosynthetic process; regulation of dendrite development; positive regulation of multicellular organism growth; protein amino acid phosphorylation; somatic muscle development; regulation of protein import into nucleus; regulation of cell shape; sequestering of lipid; myoblast fusion; negative regulation of synaptic growth at neuromuscular junction; positive regulation of axon regeneration; multicellular organism growth; regulation of multicellular organism growth; regulation of organ growth; positive regulation of cell growth; positive regulation of organ growth; epithelial cell migration, open tracheal system; dendrite regeneration; regulation of hemocyte proliferation; open tracheal system development; insulin receptor signaling pathway; lipid metabolic process; response to oxidative stress; regulation of cell size; negative regulation of apoptosis