Goat anti-Human AKR1A1 Polyclonal Antibody | anti-AKR1A1 antibody

AKR1A1, CT (Alcohol Dehydrogenase [NADP+], Aldehyde Reductase, Aldo-keto Reductase Family 1 Member A1, ALDR1, ALR)

Cat. Num. AAA643988

• Gene Names: AKR1A1; ALR; ARM; DD3; ALDR1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: AKR1A1; Polyclonal Antibody; CT (Alcohol Dehydrogenase [NADP+]; Aldehyde Reductase; Aldo-keto Reductase Family 1 Member A1; ALDR1; ALR); Anti -AKR1A1; anti-AKR1A1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human AKR1A1. Species sequence homology: Mouse, porcine and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-AKR1A1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:16,000; Western Blot: 0.5-2ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 2.5ug/ml
• Immunogen: Synthetic peptide corresponding to C-DAGHPLYPFNDPY from the C-terminal of human AKR1A1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Kidney (formalin-fixed, paraffin-embedded) stained with AKR1A1 antibody at 2.5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Antibody staining (1ug/ml) of Human Placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Testing Data

(HEK293 overexpressing AKR1A1 (RC200302) and probed with the antibody (mock transfection in first lane).)

Related Product Information for anti-AKR1A1 antibody

The AKR1A1 gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Alternative splicing of this gene results in two transcript variants encoding the same protein.

Product Categories/Family for anti-AKR1A1 antibody

Antibodies; Abs to Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 320202988
• NCBI GeneID: 10327
• NCBI Accession #: NP_001189342.1
• NCBI GenBank Nucleotide #: NM_001202413.1
• UniProt Accession #: P14550; Q6IAZ4; A8KAL8; D3DQ04
• Molecular Weight: 36,573 Da
• NCBI Official Full Name: alcohol dehydrogenase
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member A1 (aldehyde reductase)
• NCBI Official Symbol: AKR1A1
• NCBI Official Synonym Symbols: ALR; ARM; DD3; ALDR1
• NCBI Protein Information: alcohol dehydrogenase [NADP(+)]; alcohol dehydrogenase [NADP(+)]; aldehyde reductase; dihydrodiol dehydrogenase 3; aldo-keto reductase family 1 member A1
• UniProt Protein Name: Alcohol dehydrogenase [NADP(+)]
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: AKR1A1
• UniProt Synonym Gene Names: ALDR1; ALR
• UniProt Entry Name: AK1A1_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein. [provided by RefSeq, Jan 2011]

Uniprot Description

AKR1A1: Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4- nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D- glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; EC 1.1.1.2; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 1p33-p32

Cellular Component: extracellular space; apical plasma membrane; cytosol

Molecular Function: L-glucuronate reductase activity; electron carrier activity; aldehyde reductase activity

Biological Process: aldehyde catabolic process; L-ascorbic acid biosynthetic process; D-glucuronate catabolic process; glucose metabolic process; aldehyde metabolic process