Western Blot (WB)
(AKAP8L Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the AKAP8L antibody detected the AKAP8L protein (arrow).)
Rabbit anti-Human, Mouse AKAP8L Polyclonal Antibody | anti-AKAP8L antibody
AKAP8L, CT (AKAP8L, NAKAP, NAKAP95, A-kinase anchor protein 8-like, Helicase A-binding protein 95, Homologous to AKAP95 protein, Neighbor of A-kinase-anchoring protein 95) (Azide free) (HRP)
Gene Names
AKAP8L; HA95; HAP95; NAKAP; NAKAP95
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
AKAP8L; Polyclonal Antibody; CT (AKAP8L; NAKAP; NAKAP95; A-kinase anchor protein 8-like; Helicase A-binding protein 95; Homologous to AKAP95 protein; Neighbor of A-kinase-anchoring protein 95) (Azide free) (HRP); Neighbor of A-kinase-anchoring protein 95; anti-AKAP8L antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-AKAP8L antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
AKAP8L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-512 amino acids from the C-terminal region of human AKAP8L.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(AKAP8L Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the AKAP8L antibody detected the AKAP8L protein (arrow).)
Western Blot (WB)
(AKAP8L Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the AKAP8L antibody detected the AKAP8L protein (arrow).)
Western Blot (WB)
(AKAP8L Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the AKAP8L antibody detected the AKAP8L protein (arrow).)
Western Blot (WB)
(AKAP8L Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the AKAP8L antibody detected the AKAP8L protein (arrow).)
Related Product Information for anti-AKAP8L antibody
AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta.
Product Categories/Family for anti-AKAP8L antibody
References
Sugiura, T., et al. Exp. Cell Res. 314(7):1519-1528(2008). Kvissel, A.K., et al. Exp. Cell Res. 313(13):2795-2809(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Olsen, J.V., et al. Cell 127(3):635-648(2006). Sayer, J.A., et al. Neuromolecular Med. 7(4):297-310(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71649
NCBI Official Full Name
A-kinase anchor protein 8-like
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 8-like
NCBI Official Symbol
AKAP8L
NCBI Official Synonym Symbols
HA95; HAP95; NAKAP; NAKAP95
NCBI Protein Information
A-kinase anchor protein 8-like
UniProt Protein Name
A-kinase anchor protein 8-like
Protein Family
UniProt Gene Name
AKAP8L
UniProt Synonym Gene Names
NAKAP; NAKAP95; AKAP8-like protein; HAP95; HA95; Neighbor of AKAP95
UniProt Entry Name
AKP8L_HUMAN
Uniprot Description
AKAP8L: Could play a role in constitutive transport element (CTE)-mediated gene expression. Does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May regulate the initiation phase of DNA replication when associated with TMPO-beta. Belongs to the AKAP95 family.
Protein type: RNA-binding; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 19p13.12
Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nucleus
Molecular Function: protein binding; DEAD/H-box RNA helicase binding; DNA binding; metal ion binding
Research Articles on AKAP8L
Similar Products
Product Notes
The AKAP8L akap8l (Catalog #AAA6272491) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKAP8L, CT (AKAP8L, NAKAP, NAKAP95, A-kinase anchor protein 8-like, Helicase A-binding protein 95, Homologous to AKAP95 protein, Neighbor of A-kinase-anchoring protein 95) (Azide free) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AKAP8L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AKAP8L akap8l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKAP8L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
