Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12 antibody at 1:200 dilution.)
Rabbit AKAP12 Polyclonal Antibody | anti-AKAP12 antibody
AKAP12 Antibody
Gene Names
AKAP12; SSeCKS; AKAP250
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
AKAP12; Polyclonal Antibody; AKAP12 Antibody; Affinity Purified Rabbit Polyclonal AKAP12 Antibody; Polyclonal AKAP12 antibody; Anti-AKAP12 antibody; AKAP-12 antibody; A-kinase anchor protein 250kDa antibody; AKAP 12; DKFZp686M0430 antibody; AKAP250 antibody; AKAP12 antibody; FLJ20945 antibody; A-kinase anchor protein 12 antibody; AKAP-12; DKFZp686O0331 antibody; AKAP 12 antibody; Src-Suppressed C Kinase Substrate antibody; myasthenia gravis autoantigen gravin antibody; FLJ97621 antibody; anti-AKAP12 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.73 mg/ml (varies by lot)
Sequence Length
1782
Applicable Applications for anti-AKAP12 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:200
IHC-P: 1:100-1:250
ICC: 1:100-1:200
WB: 1:500-1:3000
IHC-P: 1:100-1:250
ICC: 1:100-1:200
WB: 1:500-1:3000
Immunogen
AKAP12 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 1719 and 1782 of AKAP12
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12 antibody at 1:200 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded serous OVCA using AKAP12 antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded serous OVCA using AKAP12 antibody at a dilution of 1:100)
Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:HeLa S3) using a 5% SDS PAGE gel and AKAP12 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:HeLa S3) using a 5% SDS PAGE gel and AKAP12 antibody at a dilution of 1:1000)
Related Product Information for anti-AKAP12 antibody
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis.
Product Categories/Family for anti-AKAP12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
192 kDa
NCBI Official Full Name
A-kinase anchor protein 12 isoform 1
NCBI Official Synonym Full Names
A-kinase anchoring protein 12
NCBI Official Symbol
AKAP12
NCBI Official Synonym Symbols
SSeCKS; AKAP250
NCBI Protein Information
A-kinase anchor protein 12
UniProt Protein Name
A-kinase anchor protein 12
Protein Family
UniProt Gene Name
AKAP12
UniProt Synonym Gene Names
AKAP250; AKAP-12; AKAP 250
UniProt Entry Name
AKA12_HUMAN
NCBI Description
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Research Articles on AKAP12
Similar Products
Product Notes
The AKAP12 akap12 (Catalog #AAA5312084) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's AKAP12 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:200 IHC-P: 1:100-1:250 ICC: 1:100-1:200 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the AKAP12 akap12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKAP12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
