Goat AKAP10 Polyclonal Antibody | anti-AKAP10 antibody

AKAP10 (A Kinase (PRKA) Anchor Protein 10, D-AKAP2, MGC9414, PRKA10, A-kinase Anchor Protein 10, Dual-specificity A-kinase Anchoring Protein 2, Mitochondrial A Kinase PPKA Anchor Protein 10, Protein Kinase A Anchoring Protein 10)

Cat. Num. AAA619416

• Gene Names: AKAP10; PRKA10; D-AKAP2; MGC9414
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: AKAP10; Polyclonal Antibody; AKAP10 (A Kinase (PRKA) Anchor Protein 10; D-AKAP2; MGC9414; PRKA10; A-kinase Anchor Protein 10; Dual-specificity A-kinase Anchoring Protein 2; Mitochondrial A Kinase PPKA Anchor Protein 10; Protein Kinase A Anchoring Protein 10); Anti -AKAP10 (A Kinase (PRKA) Anchor Protein 10; anti-AKAP10 antibody

• Host: Goat
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human AKAP10 at ~74kD in Rat Brain lysates (calculated MW of 73.8kD according to human NP_009133.2 and according to Rat NP_001108078.1). Species Crossreactivity: Bovine, canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-AKAP10 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:64,000; Western Blot: 0.3-1 g/ml
• Immunogen: Synthetic peptide corresponding to human AKAP10.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of AKAP10 in Rat Brain lysate (35μg protein in RIPA buffer) using AKAP10 antibody (1μg/ml).)

Related Product Information for anti-AKAP10 antibody

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction.

Product Categories/Family for anti-AKAP10 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 21493033
• NCBI GeneID: 11216
• NCBI Accession #: NP_009133.2
• NCBI GenBank Nucleotide #: NM_007202.2
• UniProt Accession #: O43572; Q96AJ7; B2R650; Q2XPN4
• Molecular Weight: 73,818 Da
• NCBI Official Full Name: A-kinase anchor protein 10, mitochondrial
• NCBI Official Synonym Full Names: A kinase (PRKA) anchor protein 10
• NCBI Official Symbol: AKAP10
• NCBI Official Synonym Symbols: PRKA10; D-AKAP2; MGC9414
• NCBI Protein Information: A-kinase anchor protein 10, mitochondrial; AKAP-10; D-AKAP-2; OTTHUMP00000065850; protein kinase A anchoring protein 10; protein kinase A-anchoring protein 10; a kinase anchor protein 10, mitochondrial; dual specificity A kinase-anchoring protein 2; dual-specificity A-kinase anchoring protein 2; mitochondrial A kinase PPKA anchor protein 10
• UniProt Protein Name: A-kinase anchor protein 10, mitochondrial
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP10
• UniProt Entry Name: AKA10_HUMAN

NCBI Description

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. [provided by RefSeq]

Uniprot Description

AKAP10: Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase. Genetic variations in AKAP10 are a cause of susceptibility to sudden cardiac death (SCD). Unexpected rapid natural death due to cardiovascular collapse within one hour of initial symptoms. It is usually caused by the worsening of existing heart diseases. The sudden onset of symptoms, such as chest pain and cardiac arrhythmias, particularly ventricular tachycardia, can lead to the loss of consciousness and cardiac arrest followed by biological death. Increased susceptibility to sudden cardiac death may be conferred by AKAP10 variants that are associated with markers of low vagus nerve sensitivity, e.g. fast basal heart rate and low heart rate variability.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p11.1

Cellular Component: mitochondrion; cytoplasm; plasma membrane; cytosol

Biological Process: protein localization; signal transduction; blood coagulation

Disease: Cardiac Conduction Defect