Rabbit anti-Human AK5 Polyclonal Antibody | anti-AK5 antibody

AK5 (Adenylate Kinase Isoenzyme 5, AK 5, ATP-AMP Transphosphorylase 5) (AP)

Cat. Num. AAA6369231

• Gene Names: AK5; AK6
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AK5; Polyclonal Antibody; AK5 (Adenylate Kinase Isoenzyme 5; AK 5; ATP-AMP Transphosphorylase 5) (AP); anti-AK5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AK5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-AK5 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human AK5, aa1-536 (NP_036225.2).
• Immunogen Sequence: MCSKPEDPVEYLESCLQKVKELGGCDKVKWDTFVSQEKKTLPPLNGGQSRRSFLRNVMPENSNFPYRRYDRLPPIHQFSIESDTDLSETAELIEEYEVFDPTRPRPKIILVIGGPGSGKGTQSLKIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTITEIKQKLMQIPDEEGIVIDGFPRDVAQALSFEDQICTPDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEKGLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFMEDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGELLREELASESERSKLIRDIMERGDLVPSGIVLELLKEAMVASLGDTRGFLIDGYPREVKQGEEFGRRIGDPQLVICMDCSADTMTNRLLQRSRSSLPVDDTTKTIAKRLEAYYRASIPVIAYYETKTQLHKINAEGTPEDVFLQLCTAIDSIF
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of AK5 expression in transfected 293T cell line by AK5 polyclonal antibody. Lane 1: AK5 transfected lysate (60.3kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-AK5 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Product Categories/Family for anti-AK5 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 28144899
• NCBI GeneID: 26289
• NCBI Accession #: NP_036225.2
• NCBI GenBank Nucleotide #: NM_012093.2
• UniProt Accession #: Q9Y6K8; Q5U622; Q6FH66; Q7Z4T5; Q86YS0; Q8N464; Q96EC9
• Molecular Weight: 60,315 Da
• NCBI Official Full Name: adenylate kinase isoenzyme 5 isoform 2
• NCBI Official Synonym Full Names: adenylate kinase 5
• NCBI Official Symbol: AK5
• NCBI Official Synonym Symbols: AK6
• NCBI Protein Information: adenylate kinase isoenzyme 5; AK 5; ATP-AMP transphosphorylase 5; adenylate kinase 6
• UniProt Protein Name: Adenylate kinase isoenzyme 5
• Protein Family: Adenylate kinase
• UniProt Gene Name: AK5
• UniProt Synonym Gene Names: AK 5
• UniProt Entry Name: KAD5_HUMAN

NCBI Description

This gene encodes a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the UMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AK5: Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Belongs to the adenylate kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.4.6; Nucleotide Metabolism - purine; EC 2.7.4.3; Kinase, other

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: cytoplasm; microtubule organizing center; cytosol

Molecular Function: nucleoside kinase activity; nucleoside diphosphate kinase activity; adenylate kinase activity; ATP binding

Biological Process: ATP metabolic process; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; nucleoside triphosphate biosynthetic process; pyrimidine ribonucleotide biosynthetic process; ADP biosynthetic process; nucleoside diphosphate phosphorylation; dADP biosynthetic process