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Western Blot (WB) ((0. 02ug/ml) staining of NIH/3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat AIP/ARA9 Polyclonal Antibody | anti-AIP antibody

Goat anti-AIP/ARA9 Antibody

Gene Names
AIP; ARA9; XAP2; XAP-2; FKBP16; FKBP37; SMTPHN
Reactivity
Tested: Mouse, Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
AIP/ARA9; Polyclonal Antibody; Goat anti-AIP/ARA9 Antibody; AIP; ARA9; aryl hydrocarbon receptor interacting protein; FKBP16; FKBP37; XAP2; HBV-X associated protein; aryl hydrocarbon receptor-interacting protein; AIP antibody; ARA9 antibody; aryl hydrocarbon receptor interacting protein antibody; FKBP16 antibody; FKBP37 antibody; XAP2 antibody; HBV-X associated protein antibody; aryl hydrocarbon receptor-interacting protein antibody; AIP / ARA9; anti-AIP antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Mouse, Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EARIRQKDEEDKAR
Sequence Length
330
Applicable Applications for anti-AIP antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 37kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 37.7kDa according to Human NP_003968.1 and 37.6kDa according to mouse NP_057875.1). In transfected HEK293 transiently expressing ARA9 a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 02-0. 06ug/ml.
Immunogen
Peptide with sequence C-EARIRQKDEEDKAR, from the C Terminus of the protein sequence according to NP_003968.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0. 02ug/ml) staining of NIH/3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0. 02ug/ml) staining of NIH/3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

(HEK293 overexpressing ARA9 (RC211157) and probed (mock transfection in first lane), tested by Origene.)

Western Blot (WB) (HEK293 overexpressing ARA9 (RC211157) and probed (mock transfection in first lane), tested by Origene.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,636 Da
NCBI Official Full Name
aryl hydrocarbon receptor interacting protein
NCBI Official Synonym Full Names
aryl hydrocarbon receptor interacting protein
NCBI Official Symbol
AIP
NCBI Official Synonym Symbols
ARA9; XAP2; XAP-2; FKBP16; FKBP37; SMTPHN
NCBI Protein Information
AH receptor-interacting protein
UniProt Protein Name
AH receptor-interacting protein
UniProt Gene Name
AIP
UniProt Synonym Gene Names
XAP2; AIP; XAP-2
UniProt Entry Name
AIP_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for aryl hydrocarbons and a ligand-activated transcription factor. The encoded protein is found in the cytoplasm as part of a multiprotein complex, but upon binding of ligand is transported to the nucleus. This protein can regulate the expression of many xenobiotic metabolizing enzymes. Also, the encoded protein can bind specifically to and inhibit the activity of hepatitis B virus. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

AIP: May play a positive role in AHR-mediated (aromatic hydrocarbon receptor) signaling, possibly by influencing its receptivity for ligand and/or its nuclear targeting. Defects in AIP are a cause of growth hormone-secreting pituitary adenoma (GHSPA); also known as familial isolated somatotropinomas (FIS) or isolated familial somatotropinoma (IFS) or familial somatotrophinoma or acromegaly due to pituitary adenoma. Defects in AIP are a cause of ACTH-secreting pituitary adenoma (ASPA); also known as pituitary Cushing disease. A pituary adenoma resulting in excessive production of adrenocorticotropic hormone. This leads to hypersecretion of cortisol by the adrenal glands and ACTH-dependent Cushing syndrome. Clinical manifestations of Cushing syndrome include facial and trunkal obesity, abdominal striae, muscular weakness, osteoporosis, arterial hypertension, diabetes. Defects in AIP are a cause of prolactin-secreting pituitary adenoma (PSPA); also known as prolactinoma. Prolactin-secreting pituitary adenoma is the most common type of hormonally active pituitary adenoma.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; plasma membrane

Molecular Function: aryl hydrocarbon receptor binding; protein binding; signal transducer activity; transcription coactivator activity; transcription factor binding; unfolded protein binding

Biological Process: negative regulation of cyclic-nucleotide phosphodiesterase activity; protein maturation via protein folding; protein targeting to mitochondrion; signal transduction; xenobiotic metabolic process

Disease: Pituitary Adenoma, Acth-secreting; Pituitary Adenoma, Growth Hormone-secreting; Pituitary Adenoma, Prolactin-secreting

Research Articles on AIP

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Product Notes

The AIP aip (Catalog #AAA421237) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-AIP/ARA9 Antibody reacts with Tested: Mouse, Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's AIP/ARA9 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 37kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 37.7kDa according to Human NP_003968.1 and 37.6kDa according to mouse NP_057875.1). In transfected HEK293 transiently expressing ARA9 a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 02-0. 06ug/ml. Researchers should empirically determine the suitability of the AIP aip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EARIRQKDEE DKAR. It is sometimes possible for the material contained within the vial of "AIP/ARA9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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