Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat AIF1/IBA1 Polyclonal Antibody | anti-AIF1 antibody
Goat anti-AIF1/IBA1 (isoform 1 and 3), biotinylated Antibody
Western Blot: Approx 17kDa band observed in Rat Brain lysates (calculated MW of 16.7kDa according to NP_001614.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
Uniprot Description
AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: actin filament; cytoplasm; cytosol; lamellipodium; nucleus; perikaryon; perinuclear region of cytoplasm; phagocytic cup
Molecular Function: actin filament binding; calcium ion binding
Biological Process: actin filament bundle formation; actin filament polymerization; cellular response to extracellular stimulus; cellular response to hormone stimulus; inflammatory response; microglial cell activation; negative regulation of apoptosis; negative regulation of smooth muscle cell proliferation; phagocytosis, engulfment; positive regulation of muscle hyperplasia; positive regulation of nitric oxide biosynthetic process; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of T cell proliferation; Rac protein signal transduction; response to axon injury; response to electrical stimulus; response to glucocorticoid stimulus
Research Articles on AIF1
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Product Notes
The AIF1 aif1 (Catalog #AAA423625) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-AIF1/IBA1 (isoform 1 and 3), biotinylated Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's AIF1/IBA1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 17kDa band observed in Rat Brain lysates (calculated MW of 16.7kDa according to NP_001614.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the AIF1 aif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TGPPAKKAIS ELP. It is sometimes possible for the material contained within the vial of "AIF1/IBA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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