Rabbit AIF Polyclonal Antibody | anti-AIF antibody

AIF polyclonal antibody

Cat. Num. AAA3014606

• Gene Names: AIFM1; AIF; CMTX4; COWCK; PDCD8; COXPD6
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Synonyms: AIF; Polyclonal Antibody; AIF polyclonal antibody; Apoptosis-inducing factor 1; mitochondrial; Programmed cell death protein 8; AIFM1; anti-AIF antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: AIF polyclonal antibody detects endogenous levels of AIF protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

• Applicable Applications for anti-AIF antibody: Western Blot (WB)
• Application Notes: WB: 1:2000~1:5000
• Immunogen: Synthetic peptide, corresponding to Human AIF.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of AIF polyclonal antibody at 1:5000 dilutionLane1:PC12 whole cell lysate(40ug)Lane2:CT-26 whole cell lysate(40ug)Lane3:Hela whole cell lysate(40ug)Lane4:Jurkat whole cell lysate(40ug)Lane5:The Kidney tissue lysate of Mouse(40ug)Lane6:HepG2 whole cell lysate(40ug)Lane7:HEK293T whole cell lysate(40ug))

Related Product Information for anti-AIF antibody

Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis. AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli. Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation. Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis. Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain. While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis. Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 195927004
• NCBI GeneID: 9131
• NCBI Accession #: NP_001124318.1
• NCBI GenBank Nucleotide #: NM_001130846.2
• UniProt Accession #: O95831; Q1L6K6; Q2QKE4; Q5JUZ7; Q6I9X6; Q9Y3I3; Q9Y3I4; A4QPB4; B1ALN1; B2RB08; D3DTE9
• Molecular Weight: 66,901 Da
• NCBI Official Full Name: apoptosis-inducing factor 1, mitochondrial isoform 4
• NCBI Official Synonym Full Names: apoptosis-inducing factor, mitochondrion-associated, 1
• NCBI Official Symbol: AIFM1
• NCBI Official Synonym Symbols: AIF; CMTX4; COWCK; PDCD8; COXPD6
• NCBI Protein Information: apoptosis-inducing factor 1, mitochondrial; striatal apoptosis-inducing factor; programmed cell death 8 (apoptosis-inducing factor)
• UniProt Protein Name: Apoptosis-inducing factor 1, mitochondrial
• Protein Family: Putative apoptosis-inducing factor
• UniProt Gene Name: AIFM1
• UniProt Synonym Gene Names: AIF; PDCD8
• UniProt Entry Name: AIFM1_HUMAN

NCBI Description

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]

Uniprot Description

PDCD8: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Belongs to the FAD-dependent oxidoreductase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.-.-.-; Mitochondrial; Oxidoreductase; Apoptosis

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: mitochondrion; perinuclear region of cytoplasm; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; oxidoreductase activity, acting on NADH or NADPH; electron carrier activity; DNA binding; NAD(P)H oxidase activity

Biological Process: neuron differentiation; chromosome condensation; caspase activation; mitochondrial respiratory chain complex I assembly; neuron apoptosis; cell redox homeostasis; apoptosis; positive regulation of apoptosis; DNA fragmentation during apoptosis; DNA catabolic process

Disease: Cowchock Syndrome