Rabbit anti-Human AGXT Polyclonal Antibody | anti-AGXT antibody

AGXT Antibody

Cat. Num. AAA9413137

• Gene Names: AGXT; AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Synonyms: AGXT; Polyclonal Antibody; AGXT Antibody; Alanine-glyoxylate aminotransferase; AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1; anti-AGXT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total AGXT protein.
• Form/Format: Supplied at 0.8mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 392

• Applicable Applications for anti-AGXT antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1000-1:2000; Western blotting: 1:200-1:1000
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to a region derived from 280-294 amino acids of human alanine-glyoxylate aminotransferase
• Target Name: AGXT
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGELane1: Hela cell lysateLane2: Human fetal liver tissue lysateLysates: 40 ug per lanePrimary antibody: 1/400 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 2 minutes)

Related Product Information for anti-AGXT antibody

Serine¡ªpyruvate aminotransferase is an enzyme that in humans is encoded by the AGXT gene. This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1), also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract.

Product Categories/Family for anti-AGXT antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4557289
• NCBI GeneID: 189
• NCBI Accession #: NP_000021
• NCBI GenBank Nucleotide #: NM_000030.2
• Molecular Weight: 43,010 Da
• NCBI Official Full Name: serine--pyruvate aminotransferase
• NCBI Official Synonym Full Names: alanine-glyoxylate aminotransferase
• NCBI Official Symbol: AGXT
• NCBI Official Synonym Symbols: AGT; PH1; SPT; AGT1; SPAT; TLH6; AGXT1
• NCBI Protein Information: serine--pyruvate aminotransferase
• UniProt Protein Name: Serine--pyruvate aminotransferase
• Protein Family: Serine--pyruvate aminotransferase
• UniProt Gene Name: AGXT
• UniProt Synonym Gene Names: AGT1; SPAT; SPT; AGT
• UniProt Entry Name: SPYA_HUMAN

NCBI Description

This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes, where it is involved in glyoxylate detoxification. Mutations in this gene, some of which alter subcellular targetting, have been associated with type I primary hyperoxaluria. [provided by RefSeq, Jul 2008]

Uniprot Description

AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Motility/polarity/chemotaxis; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.51; Mitochondrial; EC 2.6.1.44

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: peroxisomal matrix; mitochondrial matrix; peroxisome

Molecular Function: amino acid binding; protein binding; protein homodimerization activity; alanine-glyoxylate transaminase activity; serine-pyruvate transaminase activity; transaminase activity; pyridoxal phosphate binding; receptor binding

Biological Process: pyruvate biosynthetic process; response to cAMP; glyoxylate catabolic process; glyoxylate metabolic process; glycine biosynthetic process, by transamination of glyoxylate; response to glucocorticoid stimulus; oxalic acid secretion; L-cysteine catabolic process; L-alanine catabolic process

Disease: Hyperoxaluria, Primary, Type I