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Western Blot (WB) (Western blot analysis of AGPAT4 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). AGPAT4 (arrow) was detected using the purified Pab.)

Rabbit anti-Human AGPAT4 Polyclonal Antibody | anti-AGPAT4 antibody

AGPAT4, ID (1-acyl-sn-glycerol-3-phosphate Acyltransferase delta, 1-acylglycerol-3-phosphate O-acyltransferase 4, 1-AGP Acyltransferase 4, 1-AGPAT 4, Lysophosphatidic Acid Acyltransferase delta, LPAAT-delta, UNQ499/PRO1016) (Azide free) (HRP)

Gene Names
AGPAT4; 1-AGPAT4; dJ473J16.2; LPAAT-delta; RP3-473J16.2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Immunoaffinity chromatography.
Synonyms
AGPAT4; Polyclonal Antibody; ID (1-acyl-sn-glycerol-3-phosphate Acyltransferase delta; 1-acylglycerol-3-phosphate O-acyltransferase 4; 1-AGP Acyltransferase 4; 1-AGPAT 4; Lysophosphatidic Acid Acyltransferase delta; LPAAT-delta; UNQ499/PRO1016) (Azide free) (HRP); anti-AGPAT4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human AGPAT4.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-AGPAT4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the center region of human AGPAT4 (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AGPAT4 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). AGPAT4 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of AGPAT4 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). AGPAT4 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(AGPAT4 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (AGPAT4 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-AGPAT4 antibody
References
1. Leung, D.W. et.al., Front. Biosci. 6, D944-D953 (2001). 2. Clark, H.F., et.al., Genome Res. 13(10), 2265-2270 (2003).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,021 Da
NCBI Official Full Name
1-acyl-sn-glycerol-3-phosphate acyltransferase delta
NCBI Official Synonym Full Names
1-acylglycerol-3-phosphate O-acyltransferase 4
NCBI Official Symbol
AGPAT4
NCBI Official Synonym Symbols
1-AGPAT4; dJ473J16.2; LPAAT-delta; RP3-473J16.2
NCBI Protein Information
1-acyl-sn-glycerol-3-phosphate acyltransferase delta; 1-AGPAT 4; 1-AGP acyltransferase 4; lysophosphatidic acid acyltransferase delta; lysophosphatidic acid acyltransferase-delta (LPAAT-delta); 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphati
UniProt Protein Name
1-acyl-sn-glycerol-3-phosphate acyltransferase delta
UniProt Gene Name
AGPAT4
UniProt Synonym Gene Names
1-AGP acyltransferase 4; 1-AGPAT 4; LPAAT-delta
UniProt Entry Name
PLCD_HUMAN

NCBI Description

This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. [provided by RefSeq, Jul 2008]

Uniprot Description

AGPAT4: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: Lipid Metabolism - glycerolipid; Membrane protein, multi-pass; Transferase; Membrane protein, integral; EC 2.3.1.51; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; CDP-diacylglycerol biosynthetic process; phospholipid biosynthetic process

Research Articles on AGPAT4

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Product Notes

The AGPAT4 agpat4 (Catalog #AAA6461867) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGPAT4, ID (1-acyl-sn-glycerol-3-phosphate Acyltransferase delta, 1-acylglycerol-3-phosphate O-acyltransferase 4, 1-AGP Acyltransferase 4, 1-AGPAT 4, Lysophosphatidic Acid Acyltransferase delta, LPAAT-delta, UNQ499/PRO1016) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AGPAT4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AGPAT4 agpat4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGPAT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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