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Western Blot (WB) (Western Blot (WB) analysis of AGPAT3 pAb at 1:500 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:DLD whole cell lysate (40ug))

Rabbit AGPAT3 Polyclonal Antibody | anti-AGPAT3 antibody

AGPAT3 Polyclonal Antibody

Gene Names
AGPAT3; LPAAT3; 1-AGPAT 3; LPAAT-GAMMA1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >98% (by SDS-PAGE).
Synonyms
AGPAT3; Polyclonal Antibody; AGPAT3 Polyclonal Antibody; 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma; 1-acylglycerol-3-phosphate O-acyltransferase 3; 1-AGP acyltransferase 3; 1-AGPAT 3; Lysophosphatidic acid acyltransferase gamma; LPAAT-gamma; anti-AGPAT3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
AGPAT3 polyclonal antibody detects endogenous levels of AGPAT3 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >98% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
376
Applicable Applications for anti-AGPAT3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 122-166 of Human AGPAT3.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of AGPAT3 pAb at 1:500 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:DLD whole cell lysate (40ug))

Western Blot (WB) (Western Blot (WB) analysis of AGPAT3 pAb at 1:500 dilutionLane1:AML-12 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:DLD whole cell lysate (40ug))
Related Product Information for anti-AGPAT3 antibody
Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C16: 0-C20: 4 at the sn-1 position using C18: 1, C20: 4 or C18: 2-CoA as the acyl donor. Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18: 1 or C20: 4-CoA.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 45, 43, 36kDa
NCBI Official Full Name
1-acyl-sn-glycerol-3-phosphate acyltransferase gamma isoform 1
NCBI Official Synonym Full Names
1-acylglycerol-3-phosphate O-acyltransferase 3
NCBI Official Symbol
AGPAT3
NCBI Official Synonym Symbols
LPAAT3; 1-AGPAT 3; LPAAT-GAMMA1
NCBI Protein Information
1-acyl-sn-glycerol-3-phosphate acyltransferase gamma
UniProt Protein Name
1-acyl-sn-glycerol-3-phosphate acyltransferase gamma
UniProt Gene Name
AGPAT3
UniProt Synonym Gene Names
LPAAT3; 1-AGP acyltransferase 3; 1-AGPAT 3; LPAAT-gamma
UniProt Entry Name
PLCC_HUMAN

NCBI Description

The protein encoded by this gene is an acyltransferase that converts lysophosphatidic acid into phosphatidic acid, which is the second step in the de novo phospholipid biosynthetic pathway. The encoded protein may be an integral membrane protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AGPAT3: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - ether lipid; Membrane protein, multi-pass; Acetyltransferase; Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; Membrane protein, integral; EC 2.3.1.51

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; plasma membrane; nuclear envelope

Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; CDP-diacylglycerol biosynthetic process; phospholipid biosynthetic process

Research Articles on AGPAT3

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Product Notes

The AGPAT3 agpat3 (Catalog #AAA3007491) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGPAT3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AGPAT3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the AGPAT3 agpat3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGPAT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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