Rabbit anti-Mouse Agouti Polyclonal Antibody
Agouti
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography
Synonyms
Agouti; Polyclonal Antibody; Anti -Agouti; anti-Agouti antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Agouti. No homology is seen with AGRP, tubby, tub, TULP1 and TULP2. Species Sequence Homology: rat-90%, human, monkey-80%, porcine-73%, fox-75%, bovine-66%.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Form/Format
Supplied as a liquid in PBS, 0.1% BSA.
Applicable Applications for anti-Agouti antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot and ELISA.
Dilution: Western Blot: 1-10ug/ml (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well.
Optimal dilutions to be determined by researcher.
Dilution: Western Blot: 1-10ug/ml (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well.
Optimal dilutions to be determined by researcher.
Immunogen
Synthetic peptied consisting of 21aa near the N-terminus of mouse Agouti (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Agouti antibody
Several common diseases such as type II diabetes, hypertension, cardiovascular diseases, hyperlipidemia and some cancers are associated with obesity. An abnormal increase in body fat relative to lean tissue mass has been used as an indicator of obesity. High fat diet, certain environmental factors, and genetic linkage are the primary causes of obesity. In order to understand the genetic basis of obesity, several monogenic murine obesity models have been characterized including obese (Ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub). More recently, Tub, the human homolog of mouse Tub, TULP1 & TULP2 (for Tubby Like Proteins) and Agouti related protein (AGRP) have been cloned. The obesity associated with Ay mice may be due to ectopic expression of a secreted protein Agouti. Agouti protein (132aa in human) is normally expressed in skin. Its ubiquitous expression, such as in rare allele lethal yellow, causes yellow coat., linear growth, immune defects and obesity. Agouti is a paracrine-signaling molecule that affects pigmentation by inhibiting the melanocortin receptor 1 (MCR-1 and possibly MC2R and MC4R).
Product Categories/Family for anti-Agouti antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Agouti (Catalog #AAA618407) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Agouti reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Agouti can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot and ELISA. Dilution: Western Blot: 1-10ug/ml (ECL). ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Agouti for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Agouti, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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