Rabbit Aggrecan Polyclonal Antibody | anti-ACAN antibody

Aggrecan Rabbit Polyclonal

Cat. Num. AAA766445

• Gene Names: ACAN; AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: Aggrecan; Polyclonal Antibody; Aggrecan Rabbit Polyclonal; anti-ACAN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 721

• Applicable Applications for anti-ACAN antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Aggrecan
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human cartilage tissue slide using MBS766445 (ACAN Antibody) at dilution of 1:100)

Western Blot (WB)

(ROS1728 cells were subjected to SDS PAGE followed by western blot with MBS766445 (ACAN Antibody) at dilution of 1:600)

NCBI and Uniprot Product Information

• NCBI GI #: 22209083
• NCBI GeneID: 176
• Molecular Weight: 239,235 Da
• NCBI Official Full Name: Aggrecan
• NCBI Official Synonym Full Names: aggrecan
• NCBI Official Symbol: ACAN
• NCBI Official Synonym Symbols: AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP
• NCBI Protein Information: aggrecan core protein
• UniProt Protein Name: Aggrecan core protein
• Protein Family: Aggrecan core protein
• UniProt Gene Name: ACAN
• UniProt Synonym Gene Names: AGC1; CSPG1; MSK16; CSPCP; Chondroitin sulfate proteoglycan 1
• UniProt Entry Name: PGCA_HUMAN

NCBI Description

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACAN: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. Defects in ACAN are the cause of spondyloepiphyseal dysplasia type Kimberley (SEDK). Spondyloepiphyseal dysplasias are a heterogeneous group of congenital chondrodysplasias that specifically affect epiphyses and vertebrae. The autosomal dominant SEDK is associated with premature degenerative arthropathy. Defects in ACAN are the cause of spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN). A bone disease characterized by severe short stature, macrocephaly, severe midface hypoplasia, short neck, barrel chest and brachydactyly. The radiological findings comprise long bones with generalized irregular epiphyses with widened metaphyses, especially at the knees, platyspondyly, and multiple cervical-vertebral clefts. Defects in ACAN are the cause of osteochondritis dissecans short stature and early-onset osteoarthritis (OD). It is a type of osteochondritis defined as a separation of cartilage and subchondral bone from the surrounding tissue, primarily affecting the knee, ankle and elbow joints. It is clinically characterized by multiple osteochondritic lesions in knees and/or hips and/or elbows, disproportionate short stature and early-onset osteoarthritis. Belongs to the aggrecan/versican proteoglycan family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted; Extracellular matrix; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: extracellular matrix; extracellular region; Golgi lumen; lysosomal lumen; proteinaceous extracellular matrix

Molecular Function: carbohydrate binding; extracellular matrix structural constituent; hyaluronic acid binding; metal ion binding; protein binding

Biological Process: cell adhesion; central nervous system development; extracellular matrix disassembly; extracellular matrix organization and biogenesis; keratan sulfate biosynthetic process; keratan sulfate catabolic process; proteolysis; skeletal development

Disease: Osteochondritis Dissecans, Short Stature, And Early-onset Osteoarthritis; Spondyloepimetaphyseal Dysplasia, Aggrecan Type; Spondyloepiphyseal Dysplasia, Kimberley Type