Rabbit anti-Human AGFG1 Polyclonal Antibody | anti-AGFG1 antibody

AGFG1, NT (AGFG1, HRB, RAB, RIP, Arf-GAP domain and FG repeat-containing protein 1, HIV-1 Rev-binding protein, Nucleoporin-like protein RIP, Rev-interacting protein, Rev/Rex activation domain-binding protein) (PE)

Cat. Num. AAA6272211

• Gene Names: AGFG1; HRB; RAB; RIP
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AGFG1; Polyclonal Antibody; NT (AGFG1; HRB; RAB; RIP; Arf-GAP domain and FG repeat-containing protein 1; HIV-1 Rev-binding protein; Nucleoporin-like protein RIP; Rev-interacting protein; Rev/Rex activation domain-binding protein) (PE); Rev/Rex activation domain-binding protein; anti-AGFG1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 562

• Applicable Applications for anti-AGFG1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AGFG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human AGFG1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(AGFG1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the AGFG1 antibody detected the AGFG1 protein (arrow).)

Related Product Information for anti-AGFG1 antibody

The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-AGFG1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Bowes, J.D., et al. Pharmacogenet. Genomics 19(4):319-323(2009). Chaineau, M., et al. J. Biol. Chem. 283(49):34365-34373(2008). Pryor, P.R., et al. Cell 134(5):817-827(2008). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 38570132
• NCBI GeneID: 3267
• NCBI Accession #: NP_004495.2; NP_001128661.1; NP_001128660.1
• NCBI GenBank Nucleotide #: NP_004495.2
• UniProt Accession #: P52594
• NCBI Official Full Name: arf-GAP domain and FG repeat-containing protein 1 isoform 2
• NCBI Official Synonym Full Names: ArfGAP with FG repeats 1
• NCBI Official Symbol: AGFG1
• NCBI Official Synonym Symbols: HRB; RAB; RIP
• NCBI Protein Information: arf-GAP domain and FG repeat-containing protein 1
• UniProt Protein Name: Arf-GAP domain and FG repeat-containing protein 1
• Protein Family: Arf-GAP domain and FG repeat-containing protein
• UniProt Gene Name: AGFG1
• UniProt Synonym Gene Names: HRB; RAB; RIP
• UniProt Entry Name: AGFG1_HUMAN

NCBI Description

The protein encoded by this gene is related to nucleoporins, a class of proteins that mediate nucleocytoplasmic transport. The encoded protein binds the activation domain of the human immunodeficiency virus Rev protein when Rev is assembled onto its RNA target, and is required for the nuclear export of Rev-directed RNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

RIP: Required for vesicle docking or fusion during acrosome biogenesis. May play a role in RNA trafficking or localization. In case of infection by HIV-1, acts as a cofactor for viral Rev and promotes movement of Rev-responsive element- containing RNAs from the nuclear periphery to the cytoplasm. This step is essential for HIV-1 replication. Interacts with EPS15R and EPS15. Interacts with FCHO1. Ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Nuclear export

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: cell projection; cell soma; cytoplasmic membrane-bound vesicle; intracellular membrane-bound organelle; nuclear pore

Molecular Function: DNA binding; GTPase activator activity; metal ion binding; protein binding; RNA binding

Biological Process: acrosome formation; intermediate filament organization; mRNA export from nucleus; multicellular organismal development; positive regulation of GTPase activity; spermatid nuclear differentiation