Rabbit AGER Polyclonal Antibody | anti-AGER antibody

AGER Polyclonal Antibody

Cat. Num. AAA3008002

• Gene Names: AGER; RAGE; SCARJ1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AGER; Polyclonal Antibody; AGER Polyclonal Antibody; Receptor for advanced glycosylation end products; Advanced glycosylation end product-specific receptor; RAGE; anti-AGER antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AGER polyclonal antibody detects endogenous levels of AGER protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 347

• Applicable Applications for anti-AGER antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human AGER.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of mouse lung cell line, using AGER antibody.)

Related Product Information for anti-AGER antibody

Advanced glycosylation end products of proteins (AGEs) are nonenzymatically glycosylated proteins that are associated with a variety of conditions, including diabetes and other vascular disorders, as well as amyloidosis. These proteins regulate cellular functions via specific cell surface acceptor molecules, such as RAGE (receptor for advanced glycosylation end products). RAGE is a type 1 membrane protein that is found on the surface of endothelial cells, mononuclear phagocytes and vascular smooth muscle cells. Binding of AGEs to RAGE results in the induction of cellular oxidant stress and activation of the transcription factor NFkB. Evidence suggests that the induction of oxidant stress results in the activation of an intracellular cascade involving p21 ras and MAP kinase, which leads to activation of transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 332800988
• NCBI GeneID: 177
• NCBI Accession #: NP_001193895.1
• NCBI GenBank Nucleotide #: NP_001193895.1
• UniProt Accession #: Q15109
• Molecular Weight: ~43kDa
• NCBI Official Full Name: advanced glycosylation end product-specific receptor isoform 6
• NCBI Official Synonym Full Names: advanced glycosylation end-product specific receptor
• NCBI Official Symbol: AGER
• NCBI Official Synonym Symbols: RAGE; SCARJ1
• NCBI Protein Information: advanced glycosylation end product-specific receptor
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• Protein Family: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: AGER
• UniProt Synonym Gene Names: RAGE
• UniProt Entry Name: RAGE_HUMAN

NCBI Description

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

Uniprot Description

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Motility/polarity/chemotaxis; Cell cycle regulation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: cell surface receptor linked signal transduction; response to wounding; innate immune response; inflammatory response; neurite development; induction of positive chemotaxis; activation of NF-kappaB transcription factor