Western Blot (WB)
(Western blot (WB) analysis of AGBL4 polyclonal antibody at 1:500 dilutionLaneA: CT26 whole cell lysateLaneB: PC12 whole cell lysateLaneC: Hela whole cell lysateLaneD: COS-7 whole cell lysateLaneE: PC3 whole cell lysate)
Rabbit AGBL4 Polyclonal Antibody | anti-AGBL4 antibody
AGBL4 polyclonal antibody
Gene Names
AGBL4; CCP6
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Synonyms
AGBL4; Polyclonal Antibody; AGBL4 polyclonal antibody; Cytosolic carboxypeptidase 6; ATP/GTP-binding protein-like 4; CCP6; anti-AGBL4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
AGBL4 polyclonal antibody detects endogenous levels of AGBL4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-AGBL4 antibody
Western Blot (WB)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:200
IHC: 1:50~1:200
Immunogen
Synthetic peptide, corresponding to Human AGBL4
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western blot (WB) analysis of AGBL4 polyclonal antibody at 1:500 dilutionLaneA: CT26 whole cell lysateLaneB: PC12 whole cell lysateLaneC: Hela whole cell lysateLaneD: COS-7 whole cell lysateLaneE: PC3 whole cell lysate)
Western Blot (WB)
(Western blot (WB) analysis of AGBL4 polyclonal antibody at 1:500 dilutionLaneA: CT26 whole cell lysateLaneB: PC12 whole cell lysateLaneC: Hela whole cell lysateLaneD: COS-7 whole cell lysateLaneE: PC3 whole cell lysate)
Related Product Information for anti-AGBL4 antibody
Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes polyglutamates from the carboxy-terminus of target proteins such as MYLK. Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,230 Da
NCBI Official Full Name
cytosolic carboxypeptidase 6
NCBI Official Synonym Full Names
ATP/GTP binding protein-like 4
NCBI Official Symbol
AGBL4
NCBI Official Synonym Symbols
CCP6
NCBI Protein Information
cytosolic carboxypeptidase 6; ATP/GTP-binding protein-like 4
UniProt Protein Name
Cytosolic carboxypeptidase 6
UniProt Gene Name
AGBL4
UniProt Synonym Gene Names
CCP6
UniProt Entry Name
CBPC6_HUMAN
Uniprot Description
AGBL4: Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; EC 3.4.17.-
Chromosomal Location of Human Ortholog: 1p33
Cellular Component: centriole; Golgi apparatus; cytosol
Molecular Function: tubulin binding; zinc ion binding; metallocarboxypeptidase activity
Biological Process: proteolysis
Research Articles on AGBL4
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Product Notes
The AGBL4 agbl4 (Catalog #AAA3014808) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AGBL4 polyclonal antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AGBL4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500~1:1000 IHC: 1:50~1:200. Researchers should empirically determine the suitability of the AGBL4 agbl4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AGBL4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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