Rabbit anti-Human, Mouse AFF4 Polyclonal Antibody | anti-AFF4 antibody

AFF4 (AF5q31, AF5Q31, MCEF, AF4/FMR2 Family Member 4, ALL1-fused Gene from Chromosome 5q31 Protein, Major CDK9 Elongation Factor-associated Protein) (Biotin)

Cat. Num. AAA6368980

• Gene Names: AFF4; MCEF; CHOPS; AF5Q31
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AFF4; Polyclonal Antibody; AFF4 (AF5q31; AF5Q31; MCEF; AF4/FMR2 Family Member 4; ALL1-fused Gene from Chromosome 5q31 Protein; Major CDK9 Elongation Factor-associated Protein) (Biotin); anti-AFF4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AFF4. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 1753

• Applicable Applications for anti-AFF4 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human AFF4, aa1-365 (AAH25700.1).
• Immunogen Sequence: MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQRLK
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AFF4 rabbit polyclonal antibody. Western Blot analysis of AFF4 expression in human liver.)

Western Blot (WB)

(AFF4 rabbit polyclonal antibody. Western Blot analysis of AFF4 expression in mouse liver.)

Western Blot (WB)

(Western Blot analysis of AFF4 expression in transfected 293T cell line by AFF4 polyclonal antibody. Lane 1: AFF4 transfected lysate (40.2kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-AFF4 antibody

Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.

Product Categories/Family for anti-AFF4 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 19343928
• NCBI GeneID: 27125
• NCBI Official Full Name: Homo sapiens AF4/FMR2 family, member 4, mRNA
• NCBI Official Synonym Full Names: AF4/FMR2 family member 4
• NCBI Official Symbol: AFF4
• NCBI Official Synonym Symbols: MCEF; CHOPS; AF5Q31
• NCBI Protein Information: AF4/FMR2 family member 4
• Protein Family: AF4/FMR2 family

NCBI Description

The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011]