Rabbit anti-Human AF4 Polyclonal Antibody | anti-AFF1 antibody

AF4, ID (MLLT2, AF-4 protein, Proto-oncogene AF4, FEL protein)

Cat. Num. AAA6009029

• Gene Names: AFF1; AF4; PBM1; MLLT2
• Reactivity: Human
• Applications: ELISA
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: AF4; Polyclonal Antibody; ID (MLLT2; AF-4 protein; Proto-oncogene AF4; FEL protein); Anti -AF4; anti-AFF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-AFF1 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA; Dilution: ELISA: 1:1,000
• Immunogen: AF4 (MLLT2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human MLLT2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-AFF1 antibody

MLLT2 is involved in acute leukemias through a chromosomal translocation t(4;11)(q21;q23) that involves mllt2 and mll/hrx. AF-4 (MLLT2), AF-9, and ENL proteins contain nuclear targeting sequences as well as serine-rich and proline-rich regions. Stretches abundant in basic amino acids are also present in the three proteins. These results suggest that the different proteins fused to ALL-1 polypeptide(s) provide similar functional domains. AF4 is a serine- and proline-rich putative transcription factor with a glutamine-rich carboxyl terminus. The composition of the complete MLL-AF4 fusion product argues that it may act through either a gain-of-function or a dominant negative mechanism in leukemogenesis.

Product Categories/Family for anti-AFF1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 262359911
• NCBI GeneID: 4299
• NCBI Accession #: NP_001160165.1
• NCBI GenBank Nucleotide #: NM_001166693.1
• UniProt Accession #: P51825; B4DTU1; E9PBM3
• Molecular Weight: 131,422 Da
• NCBI Official Full Name: AF4/FMR2 family member 1 isoform 1
• NCBI Official Synonym Full Names: AF4/FMR2 family, member 1
• NCBI Official Symbol: AFF1
• NCBI Official Synonym Symbols: AF4; PBM1; MLLT2
• NCBI Protein Information: AF4/FMR2 family member 1; protein AF-4; proto-oncogene AF4; pre-B-cell monocytic leukemia partner 1; ALL1-fused gene from chromosome 4 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2
• UniProt Protein Name: AF4/FMR2 family member 1
• Protein Family: AF4/FMR2 family
• UniProt Gene Name: AFF1
• UniProt Synonym Gene Names: AF4; FEL; MLLT2; PBM1; Protein AF-4
• UniProt Entry Name: AFF1_HUMAN

NCBI Description

This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

AF-4: A chromosomal aberration involving AFF1 is associated with acute leukemias. Translocation t(4;11)(q21;q23) with MLL/HRX. The result is a rogue activator protein. Belongs to the AF4 family.

Protein type: Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: transcription elongation factor complex

Molecular Function: protein binding; transcription factor activity

Biological Process: positive regulation of transcription, DNA-dependent