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Western Blot (WB) (Western blot analysis of anti-ADRB2 Antibody (T384) in SK-BR-3 cell line lysates (35ug/lane). ADRB2 (arrow) was detected using the purified Pab.)

Rabbit anti-Human ADRB2 Polyclonal Antibody | anti-ADRB2 antibody

ADRB2 (T384) (ADRB2R, ADRBR, B2AR, BAR, Beta-2 Adrenergic Receptor, Beta-2 Adrenoceptor, Beta-2 Adrenoreceptor, BETA2AR, Adrenergic, beta-2-, Receptor, Surface) (Azide free) (HRP)

Gene Names
ADRB2; BAR; B2AR; ADRBR; ADRB2R; BETA2AR
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Immunoaffinity chromatography.
Synonyms
ADRB2; Polyclonal Antibody; ADRB2 (T384) (ADRB2R; ADRBR; B2AR; BAR; Beta-2 Adrenergic Receptor; Beta-2 Adrenoceptor; Beta-2 Adrenoreceptor; BETA2AR; Adrenergic; beta-2-; Receptor; Surface) (Azide free) (HRP); anti-ADRB2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human BAR2.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-ADRB2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to amino acid residues surrounding T384 of human BAR2 (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ADRB2 Antibody (T384) in SK-BR-3 cell line lysates (35ug/lane). ADRB2 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-ADRB2 Antibody (T384) in SK-BR-3 cell line lysates (35ug/lane). ADRB2 (arrow) was detected using the purified Pab.)
Product Categories/Family for anti-ADRB2 antibody
References
1. Kobilka B.K., Dixon R.A.F.Proc. Natl. Acad. Sci. USA 84:46-50(1987) 2. Emorine L.J., Marullo S.Proc. Natl. Acad. Sci. USA 84:6995-6999(1987) 3. Kobilka B.K., Frielle T.J. Biol. Chem. 262:7321-7327(1987).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
154
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,459 Da
NCBI Official Full Name
beta-2 adrenergic receptor
NCBI Official Synonym Full Names
adrenoceptor beta 2, surface
NCBI Official Symbol
ADRB2
NCBI Official Synonym Symbols
BAR; B2AR; ADRBR; ADRB2R; BETA2AR
NCBI Protein Information
beta-2 adrenergic receptor; adrenergic, beta-2-, receptor, surface; adrenoceptor beta 2 surface; beta-2 adrenoceptor; beta-2 adrenoreceptor; catecholamine receptor
UniProt Protein Name
Beta-2 adrenergic receptor
UniProt Gene Name
ADRB2
UniProt Synonym Gene Names
ADRB2R; B2AR; Beta-2 adrenoceptor
UniProt Entry Name
ADRB2_HUMAN

Uniprot Description

ADRB2: Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31-q32

Cellular Component: integral to plasma membrane; lysosome; apical plasma membrane; plasma membrane; nucleus; endosome; receptor complex

Molecular Function: protein binding; protein homodimerization activity; potassium channel regulator activity; beta2-adrenergic receptor activity; norepinephrine binding; adenylate cyclase binding

Biological Process: arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; diet induced thermogenesis; negative regulation of multicellular organism growth; transmembrane receptor protein tyrosine kinase activation (dimerization); regulation of vasodilation; adenylate cyclase activation; regulation of sodium ion transport; positive regulation of bone mineralization; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; positive regulation of protein ubiquitination; heat generation; negative regulation of smooth muscle contraction; brown fat cell differentiation; endosome to lysosome transport; positive regulation of transcription from RNA polymerase II promoter; response to cold; bone resorption; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure

Disease: Obesity; Asthma, Susceptibility To

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Product Notes

The ADRB2 adrb2 (Catalog #AAA6461812) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADRB2 (T384) (ADRB2R, ADRBR, B2AR, BAR, Beta-2 Adrenergic Receptor, Beta-2 Adrenoceptor, Beta-2 Adrenoreceptor, BETA2AR, Adrenergic, beta-2-, Receptor, Surface) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADRB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ADRB2 adrb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADRB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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