Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of ADPRHL2 Antibody (N-term) in Hela cell line lysates (35ug/lane). ADPRHL2 (arrow) was detected using the purified Pab.)

Rabbit anti-Human ADPRHL2 Polyclonal Antibody | anti-ADPRHL2 antibody

ADPRHL2, NT (ADPRHL2, ARH3, Poly(ADP-ribose) glycohydrolase ARH3, ADP-ribosylhydrolase 3, [Protein ADP-ribosylarginine] hydrolase-like protein 2) (FITC)

Gene Names
ADPRHL2; ARH3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ADPRHL2; Polyclonal Antibody; NT (ADPRHL2; ARH3; Poly(ADP-ribose) glycohydrolase ARH3; ADP-ribosylhydrolase 3; [Protein ADP-ribosylarginine] hydrolase-like protein 2) (FITC); [Protein ADP-ribosylarginine] hydrolase-like protein 2; anti-ADPRHL2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-ADPRHL2 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ADPRHL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~123 amino acids from the N-terminal region of human ADPRHL2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ADPRHL2 Antibody (N-term) in Hela cell line lysates (35ug/lane). ADPRHL2 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ADPRHL2 Antibody (N-term) in Hela cell line lysates (35ug/lane). ADPRHL2 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(ADPRHL2 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ADPRHL2 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ADPRHL2 antibody
ADPRHL2 is a member of the ADP-ribosylglycohydrolase family. The enzyme catalyzes the removal of ADP-ribose from ADP-ribosylated proteins. This enzyme localizes to the mitochondria, in addition to the nucleus and cytoplasm.
Product Categories/Family for anti-ADPRHL2 antibody
References
Niere, M., et al. Mol. Cell. Biol. 28(2):814-824(2008). Ono, T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(45):16687-16691(2006). Mueller-Dieckmann, C., et al. Proc. Natl. Acad. Sci. U.S.A. 103(41):15026-15031(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,947 Da
NCBI Official Full Name
poly(ADP-ribose) glycohydrolase ARH3
NCBI Official Synonym Full Names
ADP-ribosylhydrolase like 2
NCBI Official Symbol
ADPRHL2
NCBI Official Synonym Symbols
ARH3
NCBI Protein Information
poly(ADP-ribose) glycohydrolase ARH3; poly(ADP-ribose) glycohydrolase ARH3; ADP-ribosylhydrolase 3; [Protein ADP-ribosylarginine] hydrolase-like protein 2; protein ADP-ribosylarginine hydrolase-like protein 2
UniProt Protein Name
Poly(ADP-ribose) glycohydrolase ARH3
UniProt Gene Name
ADPRHL2
UniProt Synonym Gene Names
ARH3
UniProt Entry Name
ARHL2_HUMAN

NCBI Description

This gene encodes a member of the ADP-ribosylglycohydrolase family. The encoded enzyme catalyzes the removal of ADP-ribose from ADP-ribosylated proteins. This enzyme localizes to the mitochondria, in addition to the nucleus and cytoplasm.[provided by RefSeq, Feb 2009]

Uniprot Description

ADPRHL2: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. Generates ADP-ribose from poly-(ADP-ribose), but does not hydrolyze ADP- ribose-arginine, -cysteine, -diphthamide, or -asparagine bonds. Belongs to the ADP-ribosylglycohydrolase family.

Protein type: EC 3.2.1.143; Hydrolase

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; mitochondrial matrix

Molecular Function: metal ion binding; poly(ADP-ribose) glycohydrolase activity

Biological Process: metabolic process

Research Articles on ADPRHL2

Similar Products

Product Notes

The ADPRHL2 adprhl2 (Catalog #AAA6271885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADPRHL2, NT (ADPRHL2, ARH3, Poly(ADP-ribose) glycohydrolase ARH3, ADP-ribosylhydrolase 3, [Protein ADP-ribosylarginine] hydrolase-like protein 2) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADPRHL2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ADPRHL2 adprhl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADPRHL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.