Rabbit anti-Human, Rat ADP Ribosylation Factor Interacting Protein 2 Polyclonal Antibody | anti-ARFIP2 antibody
ADP Ribosylation Factor Interacting Protein 2 (ARFIP2, Arfaptin 2, Partner of RAC1, POR1)
Gene Names
ARFIP2; POR1; FLJ18046; FLJ18697; FLJ99239
Reactivity
Human, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by immunoaffinity chromatography
Synonyms
ADP Ribosylation Factor Interacting Protein 2; Polyclonal Antibody; ADP Ribosylation Factor Interacting Protein 2 (ARFIP2; Arfaptin 2; Partner of RAC1; POR1); Anti -ADP Ribosylation Factor Interacting Protein 2 (ARFIP2; anti-ARFIP2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Arfaptin 2 protein at ~38kD. Also bands observed at ~23, ~35, and ~150kD in some lysate samples. Species Crossreactivity: rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography
Purified by immunoaffinity chromatography
Form/Format
Supplied as a liquid in PBS, pH7.4, 0.1% sodium azide.
Applicable Applications for anti-ARFIP2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 0.3-7ug/ml
Western Blot: 1-3ug/ml
Dilution: Immunofluorescence: 0.3-7ug/ml
Western Blot: 1-3ug/ml
Immunogen
Synthetic peptide derived from the N-terminal region of the human Arfaptin 2 protein (ADP-ribosylation factor interacting protein 2, AFP2, Partner of Rac1, or POR1)
Positive Control
human HeLa cervical carcinoma, A549 lung carcinoma, MCF-7 breast adenocarcinoma, T47D breast ductal carcinoma, NCI-H69 lung carcinoma, rat PC12, and GFP-Arfaptin 2-transfected HeLa cell lysates.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%), aliquot and store at -20 degree C or colder. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-ARFIP2 antibody
Arfaptin 2, or ADP-ribosylation factor interacting protein 2, is a Rac1 binding protein necessary for Rac-mediated actin polymerization and the subsequent formation of membrane ruffles and lamellipodia.1 Arfaptin-2 has been shown to be involved in interactions between the Rac and ARF signaling pathways.2 Arfaptin 2 also regulates the aggregation of mutant Huntingtin protein by possibly impairing proteasome function. Expression of Arfaptin 2 was shown to be increased at sites of neurodegeneration. Mutant Huntingtin protein is characteristic of Huntington's disease, an inherited neurodegenerative disorder.3
Product Categories/Family for anti-ARFIP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
37,856 Da
NCBI Official Full Name
ADP-ribosylation factor interacting protein 2
NCBI Official Synonym Full Names
ADP-ribosylation factor interacting protein 2
NCBI Official Symbol
ARFIP2
NCBI Official Synonym Symbols
POR1; FLJ18046; FLJ18697; FLJ99239
NCBI Protein Information
arfaptin-2; OTTHUMP00000164590; OTTHUMP00000231513; partner of RAC1 (arfaptin 2)
UniProt Protein Name
Arfaptin-2
Protein Family
UniProt Gene Name
ARFIP2
UniProt Synonym Gene Names
POR1
UniProt Entry Name
ARFP2_HUMAN
Uniprot Description
arfaptin 2: Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: 11p15
Cellular Component: ruffle; cytoplasm; plasma membrane; trans-Golgi network membrane; cell cortex
Molecular Function: protein domain specific binding; protein binding; GTP binding; GTP-dependent protein binding; Rac GTPase binding
Biological Process: lamellipodium biogenesis; small GTPase mediated signal transduction; ruffle organization and biogenesis; actin cytoskeleton organization and biogenesis; cell motility
Research Articles on ARFIP2
Similar Products
Product Notes
The ARFIP2 arfip2 (Catalog #AAA630677) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADP Ribosylation Factor Interacting Protein 2 (ARFIP2, Arfaptin 2, Partner of RAC1, POR1) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ADP Ribosylation Factor Interacting Protein 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: 0.3-7ug/ml Western Blot: 1-3ug/ml. Researchers should empirically determine the suitability of the ARFIP2 arfip2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADP Ribosylation Factor Interacting Protein 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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