Rabbit anti-Human Adipose Triglyceride Lipase Polyclonal Antibody | anti-PNPLA2 antibody

Adipose Triglyceride Lipase (ATGL, Desnutrin, Phospholipase A2 zeta)

Cat. Num. AAA610316

• Gene Names: PNPLA2; ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; DKFZp667M109; 1110001C14Rik
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Adipose Triglyceride Lipase; Polyclonal Antibody; Adipose Triglyceride Lipase (ATGL; Desnutrin; Phospholipase A2 zeta); Anti -Adipose Triglyceride Lipase (ATGL; anti-PNPLA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Species Crossreactivity: This antibody reacts with human protein. Although it detects mouse protein, it does so weakly. Other species have not been tested.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-glycine, 150mM sodium chloride, pH 7.2.

• Applicable Applications for anti-PNPLA2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: A synthetic peptide within the C-terminal region [residues 400-500] of the human ATGL protein. Homology: 100% with human
• Cellular Localization: Adipocytes
• Positive Control: Human adipose tissue lysates and mouse 3T3-L1 cell lysates
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PNPLA2 antibody

Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.

Product Categories/Family for anti-PNPLA2 antibody

Antibodies; Abs to Enzymes; Phospholipase

NCBI and Uniprot Product Information

• NCBI GI #: 58759051
• NCBI GeneID: 57104
• UniProt Accession #: Q96AD5; O60643; Q5EFF5; Q6XYE5; Q96ET6; Q9NQ61; Q9NQ62
• Molecular Weight: 55,316 Da
• NCBI Official Full Name: adipose triglyceride lipase
• NCBI Official Synonym Full Names: patatin-like phospholipase domain containing 2
• NCBI Official Symbol: PNPLA2
• NCBI Official Synonym Symbols: ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; DKFZp667M109; 1110001C14Rik
• NCBI Protein Information: patatin-like phospholipase domain-containing protein 2; TTS2.2; desnutrin; IPLA2-zeta; OTTHUMP00000164432; triglyceride hydrolase; adipose triglyceride lipase; transport-secretion protein 2.2; pigment epithelium-derived factor; calcium-independent phospholipase A2
• UniProt Protein Name: Patatin-like phospholipase domain-containing protein 2
• Protein Family: Patatin-like phospholipase domain-containing protein
• UniProt Gene Name: PNPLA2
• UniProt Synonym Gene Names: ATGL
• UniProt Entry Name: PLPL2_HUMAN

NCBI Description

This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq]

Uniprot Description

PNPLA2: the rate-limiting lipolytic enzyme in mammals, flies, and yeast. Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Upregulated by exercise training in human skeletal muscle. Has acylglycerol transacylase activity. May act coordinately with HSL within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. Interacting with ABHD5 stimulates its triglyceride hydrolase activity. Despite a colocalization in lipid droplets, it probably does not interact with perilipin. Transcriptionally regulated by FOXO1A. Defects cause neutral lipid storage disease (NLSD), an autosomal recessive disorder characterized by the excessive accumulation of neutral lipids in multiple tissues. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipase; EC 3.1.1.3; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane; plasma membrane; lipid particle; cytosol

Molecular Function: triacylglycerol lipase activity

Biological Process: sequestering of lipid; phospholipid metabolic process; glycerophospholipid biosynthetic process; triacylglycerol catabolic process

Disease: Neutral Lipid Storage Disease With Myopathy