Guinea pig Adipophilin Polyclonal Antibody | anti-PLIN2 antibody

Adipophilin (ADFP, Adipose Differentiation-related Protein)

Cat. Num. AAA610278

• Gene Names: PLIN2; ADFP; ADRP; MGC10598
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Serum; Serum
• Synonyms: Adipophilin; Polyclonal Antibody; Adipophilin (ADFP; Adipose Differentiation-related Protein); Anti -Adipophilin (ADFP; anti-PLIN2 antibody

• Host: Guinea pig
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Adipophilin at ~52kD. Reactive with cell lines
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in 0.09% sodium azide.

• Applicable Applications for anti-PLIN2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applicatons not tested.; Dilution: Western Blot: 1:2000; Immunohistochemistry (Frozen, Paraffin embedded tissue after microwave treatment): 1:100; Incubate for 1hr at RT or overnight at 4 degree C.; Optimal dilutions to be determined by the reseracher.
• Immunogen: Synthetic peptide corresponding to the N-terminal (aa1-29) of human and murine Adipophilin.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PLIN2 antibody

Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir. Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. New specific marker for liposarcoma.

Product Categories/Family for anti-PLIN2 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1806040
• NCBI GeneID: 123
• UniProt Accession #: Q99541; Q9BSC3; Q5SYF3; Q5SYF5; Q6FHZ7
• Molecular Weight: 48,075 Da
• NCBI Official Full Name: adipophilin
• NCBI Official Synonym Full Names: perilipin 2
• NCBI Official Symbol: PLIN2
• NCBI Official Synonym Symbols: ADFP; ADRP; MGC10598
• NCBI Protein Information: perilipin-2; adipophilin; OTTHUMP00000021107; adipose differentiation-related protein
• UniProt Protein Name: Perilipin-2
• Protein Family: Perilipin
• UniProt Gene Name: PLIN2
• UniProt Synonym Gene Names: ADFP
• UniProt Entry Name: PLIN2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Uniprot Description

perilipin 2: May be involved in development and maintenance of adipose tissue. Belongs to the perilipin family.

Protein type: Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 9p22.1

Cellular Component: endoplasmic reticulum; plasma membrane; extracellular region; lipid particle; cytosol; nucleus

Biological Process: long-chain fatty acid transport; response to drug; sequestering of lipid; cellular lipid metabolic process; response to organic cyclic substance