Western Blot (WB)
(ADHFE1 rabbit polyclonal antibody. Western Blot analysis of ADHFE1 expression in mouse kidney.)
Rabbit anti-Human, Mouse ADHFE1 Polyclonal Antibody | anti-ADHFE1 antibody
ADHFE1 (Hydroxyacid-oxoacid Transhydrogenase, Mitochondrial, HOT, Alcohol Dehydrogenase Iron-containing Protein 1, ADHFe1, Fe-containing Alcohol Dehydrogenase, HMFT2263) APC
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ADHFE1; Polyclonal Antibody; ADHFE1 (Hydroxyacid-oxoacid Transhydrogenase; Mitochondrial; HOT; Alcohol Dehydrogenase Iron-containing Protein 1; ADHFe1; Fe-containing Alcohol Dehydrogenase; HMFT2263) APC; anti-ADHFE1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ADHFE1. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Sequence Length
419
Applicable Applications for anti-ADHFE1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human ADHFE1, aa1-419 (NP_653251.1).
Immunogen Sequence
MAVSNIRYGAAVTKEVGMDLKNMGAKNVCLMTDKNLSKLPPVQVAMDSLVKNGIPFTVYDNVRVEPTDSSFMEAIEFAQKGAFDAYVAVGGGSTMDTCKAANLYASSPHSDFLDYVSAPIGKGKPVSVPLKPLIAVPTTSGTGSETTGVAIFDYEHLKVKIGITSRAIKPTLGLIDPLHTLHMPARVVANSGFDVLCHALESYTTLPYHLRSPCPSNPITRPAYQGSNPISDIWAIHALRIVAKYLKRAVRNPDDLEARSHMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKMYKAKDYNVDHPLVPHGLSVVLTSPAVFTFTAQMFPERHLEMAEILGADTRTARIQDAGLVLADTLRKFLFDLDVDDGLAAVGYSKADIPALVKGTLPQERVTKLAPRPQSEEDLAALFEASMKLY
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(ADHFE1 rabbit polyclonal antibody. Western Blot analysis of ADHFE1 expression in mouse kidney.)
Western Blot (WB)
(ADHFE1 rabbit polyclonal antibody. Western Blot analysis of ADHFE1 expression in mouse kidney.)
Western Blot (WB)
(ADHFE1 rabbit polyclonal antibody. Western Blot analysis of ADHFE1 expression in K-562.)
Western Blot (WB)
(ADHFE1 rabbit polyclonal antibody. Western Blot analysis of ADHFE1 expression in K-562.)
Western Blot (WB)
(Western Blot analysis of ADHFE1 expression in transfected 293T cell line by ADHFE1 polyclonal antibody. Lane 1: ADHFE1 transfected lysate (45.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ADHFE1 expression in transfected 293T cell line by ADHFE1 polyclonal antibody. Lane 1: ADHFE1 transfected lysate (45.2kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-ADHFE1 antibody
Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities.
Product Categories/Family for anti-ADHFE1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
alcohol dehydrogenase, iron containing, 1 isoform 2
Protein Family
Similar Products
Product Notes
The ADHFE1 (Catalog #AAA6368924) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADHFE1 (Hydroxyacid-oxoacid Transhydrogenase, Mitochondrial, HOT, Alcohol Dehydrogenase Iron-containing Protein 1, ADHFe1, Fe-containing Alcohol Dehydrogenase, HMFT2263) APC reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ADHFE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ADHFE1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADHFE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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