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Immunohistochemistry (IHC) (Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))

Rabbit anti-Human ADH5 Polyclonal Antibody | anti-ADH5 antibody

Anti-ADH5 Antibody (aa212-239)

Gene Names
ADH5; FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH; HEL-S-60p
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Protein A purified
Synonyms
ADH5; Polyclonal Antibody; Anti-ADH5 Antibody (aa212-239); Goat Polyclonal to Human ADH5; Alcohol dehydrogenase 5; ADHX; Formaldehyde dehydrogenase; ADH-3; Alcohol dehydrogenase class-3; FALDH; GSH-FDH; GSNOR; FDH; anti-ADH5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
This ADH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-239 amino acids from the Central region of human ADH5.
Purity/Purification
Protein A purified
Form/Format
PBS, 0.09% sodium azide
Sequence Length
374
Applicable Applications for anti-ADH5 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
FC/FACS: 1:10-1:50
IHC-P: 1:10-1:100
WB: 1:1000
Conjugation
Unconjugated
Epitope
aa212-239
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC) (Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (WB)

(Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (WB) (Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot of ADH5 Antibody in K562 cell line lysates (35 ug/lane). ADH5 (arrow) was detected using the purified antibody.)

Western Blot (WB) (Western blot of ADH5 Antibody in K562 cell line lysates (35 ug/lane). ADH5 (arrow) was detected using the purified antibody.)

Western Blot (WB)

(Western blot of ADH5 (arrow) using rabbit polyclonal ADH5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.)

Western Blot (WB) (Western blot of ADH5 (arrow) using rabbit polyclonal ADH5 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ADH5 gene.)

Flow Cytometry (FC/FACS)

(Flow cytometric of K562 cells using ADH5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric of K562 cells using ADH5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ADH5 antibody
ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipidperoxidation products. The encoded protein forms a homodimer. It hasvirtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation ofS-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
128
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,724 Da
NCBI Official Full Name
alcohol dehydrogenase class-3
NCBI Official Synonym Full Names
alcohol dehydrogenase 5 (class III), chi polypeptide
NCBI Official Symbol
ADH5
NCBI Official Synonym Symbols
FDH; ADHX; ADH-3; FALDH; GSNOR; GSH-FDH; HEL-S-60p
NCBI Protein Information
alcohol dehydrogenase class-3
UniProt Protein Name
Alcohol dehydrogenase class-3
Protein Family
UniProt Gene Name
ADH5
UniProt Synonym Gene Names
FALDH; FDH; GSH-FDH

NCBI Description

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.

Research Articles on ADH5

Similar Products

Product Notes

The ADH5 adh5 (Catalog #AAA2401814) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ADH5 Antibody (aa212-239) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADH5 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). FC/FACS: 1:10-1:50 IHC-P: 1:10-1:100 WB: 1:1000. Researchers should empirically determine the suitability of the ADH5 adh5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADH5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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