Rabbit anti-Human ADH1B Polyclonal Antibody | anti-ADH1B antibody

ADH1B, ID (Alcohol Dehydrogenase 1B, Alcohol Dehydrogenase Subunit beta, ADH2) (FITC)

Cat. Num. AAA6462229

• Gene Names: ADH1B; ADH2; HEL-S-117
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ADH1B; Polyclonal Antibody; ID (Alcohol Dehydrogenase 1B; Alcohol Dehydrogenase Subunit beta; ADH2) (FITC); anti-ADH1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADH1B.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ADH1B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ADH1B (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of ADH1B Antibody (Center) in HepG2 cell line lysates (35ug/lane). ADH1B (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ADH1B Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ADH1B Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-ADH1B antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Alcohol intake, Am. J. Gastroenterol. 104(9), 2182-2188 (2009). 2. Nishimura, F.T., Nihon Arukoru Yakubutsu Igakkai Zasshi 44(3), 139-155 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 34577061
• NCBI GeneID: 125
• NCBI Accession #: NP_000659
• NCBI GenBank Nucleotide #: NM_000668.4
• UniProt Accession #: P00325; Q13711; Q4ZGI9; Q96KI7; A8MYN5; B4DRS9; B4DVC3
• Molecular Weight: 35,412 Da
• NCBI Official Full Name: alcohol dehydrogenase 1B isoform 1
• NCBI Official Synonym Full Names: alcohol dehydrogenase 1B (class I), beta polypeptide
• NCBI Official Symbol: ADH1B
• NCBI Official Synonym Symbols: ADH2; HEL-S-117
• NCBI Protein Information: alcohol dehydrogenase 1B
• UniProt Protein Name: Alcohol dehydrogenase 1B
• UniProt Gene Name: ADH1B
• UniProt Synonym Gene Names: ADH2
• UniProt Entry Name: ADH1B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Uniprot Description

ADH1B: Dimer of identical or non-identical chains of three types; alpha, beta and gamma. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: Lipid Metabolism - fatty acid; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.1; Oxidoreductase; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: zinc ion binding; alcohol dehydrogenase activity, zinc-dependent

Biological Process: xenobiotic metabolic process; ethanol oxidation

Disease: Alcohol Dependence