Immunohistochemistry (IHC)
(Anti-GPR125 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Goat ADGRA3/GPR125 Polyclonal Antibody | anti-ADGRA3/GPR125 antibody
Anti-ADGRA3/GPR125 Antibody (aa1098-1109)
Gene Names
ADGRA3; PGR21; TEM5L; GPR125
Reactivity
HumanPredicted: Rabbit, Mouse, Bovine, Hamster, Horse, Bat, Monkey
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
ADGRA3/GPR125; Polyclonal Antibody; Anti-ADGRA3/GPR125 Antibody (aa1098-1109); Rabbit Polyclonal to Human ADGRA3/GPR125; ADGRA3; GPR125; TEM5L; G protein-coupled receptor 125; PGR21; anti-ADGRA3/GPR125 antibody
Host
Goat
Reactivity
Human
Predicted: Rabbit, Mouse, Bovine, Hamster, Horse, Bat, Monkey
Predicted: Rabbit, Mouse, Bovine, Hamster, Horse, Bat, Monkey
Clonality
Polyclonal
Specificity
Human GPR125.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Sequence Length
1321
Applicable Applications for anti-ADGRA3/GPR125 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
ELISA: 1:8000
IHC-P: 3.75 ug/ml
WB: 0.5-1.5 ug/ml
IHC-P: 3.75 ug/ml
WB: 0.5-1.5 ug/ml
Immunogen
Synthetic peptide CTNKSASSFKNS from an internal region of human GPR125 (NP_660333.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Bovine, Bat, Horse, Rabbit (100%); Panda, Dog, Pig, Opossum, Turkey, Platypus, Xenopus (92%); Chicken (83%).
Conjugation
Unconjugated
Epitope
aa1098-1109
Preparation and Storage
Store at -20 degree C.
Avoid freeze-thaw cycles.
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Anti-GPR125 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC)
(Anti-GPR125 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Western Blot (WB)
(Antibody (0.5 ug/ml) staining of Human Ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
(Antibody (0.5 ug/ml) staining of Human Ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Related Product Information for anti-ADGRA3/GPR125 antibody
GPR125 is an Orphan-B GPCR with an unknown ligand. GPR125, like GPR124, has a leucine rich repeat (LRR), an immunoglobulin (Ig) domain, and ahormone-binding domain (HBD). the Ig domain shows similarities tomotilin and titin, while the LRR domain shows similarities to LRIG1 and SLIT1-2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,973 Da
NCBI Official Full Name
adhesion G protein-coupled receptor A3
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor A3
NCBI Official Symbol
ADGRA3
NCBI Official Synonym Symbols
PGR21; TEM5L; GPR125
NCBI Protein Information
adhesion G protein-coupled receptor A3
UniProt Protein Name
Adhesion G protein-coupled receptor A3
UniProt Gene Name
ADGRA3
NCBI Description
This gene encodes a member of the G protein-coupled receptor superfamily. This membrane protein may play a role in tumor angiogenesis through its interaction with the human homolog of the Drosophila disc large tumor suppressor gene. This gene is mapped to a candidate region of chromosome 4 which may be associated with bipolar disorder and schizophrenia. [provided by RefSeq, Oct 2012]
Uniprot Description
Orphan receptor that may have a role in planar cell polarity pathway.
Research Articles on ADGRA3/GPR125
Similar Products
Product Notes
The ADGRA3/GPR125 adgra3 (Catalog #AAA2400835) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-ADGRA3/GPR125 Antibody (aa1098-1109) reacts with Human Predicted: Rabbit, Mouse, Bovine, Hamster, Horse, Bat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ADGRA3/GPR125 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ELISA: 1:8000 IHC-P: 3.75 ug/ml WB: 0.5-1.5 ug/ml. Researchers should empirically determine the suitability of the ADGRA3/GPR125 adgra3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADGRA3/GPR125, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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