anti-Human Adenosine A3 Receptor Polyclonal Antibody | anti-ADORA3 antibody
Anti - Adenosine A3 Receptor (A3R)
Gene Names
ADORA3; A3AR; AD026; bA552M11.5; RP11-552M11.7
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Adenosine A3 Receptor; Polyclonal Antibody; Anti - Adenosine A3 Receptor (A3R); Adenosine A3 Receptor (A3R); anti-ADORA3 antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The human A3R peptide shows only 46% homology (7/15 AA) with rat and 20% (3/15 AA) with sheep A3R sequences. We recommend the use of A3R31 that is made to the rat A3R sequence for the detection of rat A3R. Antibody crossreactivity in various species is not established. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
8
Applicable Applications for anti-ADORA3 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10 mg/ml for affinity pure using ECL. Based upon mRNA studies, the A3R has been found in rat testis and lung, kidney, heart and brain (1). Other studies have found the mRNA to be highest in lung, spleen, and pineal glands, and to a moderate levels in brain, kidney, and testis (2). It has been found in lung, liver, kidney, and heart and very little in skeletal muscle and brain (3).
ELISA: 1:10,000-50,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10 mg/ml for affinity pure using ECL. Based upon mRNA studies, the A3R has been found in rat testis and lung, kidney, heart and brain (1). Other studies have found the mRNA to be highest in lung, spleen, and pineal glands, and to a moderate levels in brain, kidney, and testis (2). It has been found in lung, liver, kidney, and heart and very little in skeletal muscle and brain (3).
ELISA: 1:10,000-50,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Immunogen
A 15 amino acid sequence corresponding to human A3R gene (1). This region corresponds to the 3rd extracellular domain. A cysteine has been added at the NH2 terminus for coupling to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-ADORA3 antibody
References
• 1. Zhou, Q-Y, et al (1992) Proc. Natl. Acad. Sci. 89, 7432-74361; Tucker, AL and Linden, J (1993) Cardiovascular Res. 27, 62-67; Olah, ME and Stile, G (1995) Ann. Rev. Pharmacol. 35, 581-606;
• 2. Linden J et al (1993) Mol. Pharmacol. 44, 524-532;
• 3. Sajjadi, FG et al (1993) BBA 1179, 105-107
• 2. Linden J et al (1993) Mol. Pharmacol. 44, 524-532;
• 3. Sajjadi, FG et al (1993) BBA 1179, 105-107
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
847 Da
NCBI Official Full Name
adenosine A3 receptor
NCBI Official Synonym Full Names
adenosine A3 receptor
NCBI Official Symbol
ADORA3
NCBI Official Synonym Symbols
A3AR; AD026; bA552M11.5; RP11-552M11.7
NCBI Protein Information
adenosine receptor A3; OTTHUMP00000013476; OTTHUMP00000013479; OTTHUMP00000013739; OTTHUMP00000082917
UniProt Protein Name
Adenosine A3 receptor protein
Protein Family
UniProt Gene Name
adenosine A3 receptor
UniProt Entry Name
Q6LD47_HUMAN
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Product Notes
The ADORA3 adenosine a3 receptor (Catalog #AAA614953) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - Adenosine A3 Receptor (A3R) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Adenosine A3 Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10 mg/ml for affinity pure using ECL. Based upon mRNA studies, the A3R has been found in rat testis and lung, kidney, heart and brain (1). Other studies have found the mRNA to be highest in lung, spleen, and pineal glands, and to a moderate levels in brain, kidney, and testis (2). It has been found in lung, liver, kidney, and heart and very little in skeletal muscle and brain (3). ELISA: 1:10,000-50,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the ADORA3 adenosine a3 receptor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adenosine A3 Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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