Rabbit Adenosine A2a Receptor Polyclonal Antibody | anti-ADORA2A antibody

Adenosine A2a Receptor

Cat. Num. AAA630407

• Gene Names: ADORA2A; RDC8; hA2aR; ADORA2
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Adenosine A2a Receptor; Polyclonal Antibody; Anti -Adenosine A2a Receptor; anti-ADORA2A antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes canine Adenosine A2a receptor. Recognizes a band of ~45kD in human (coronary artery), and rat, porcine and bovine striatal membranes.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS,pH 7.4, 0.1% BSA, 0.05% sodium azide.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-ADORA2A antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Dilution: ELISA: 1:10,000-1:50,000; Immunohistochemistry (Frozen): 10-50ug/ml; Western Blot: 1-10ug/ml (Chemiluminescence technique), 40-100ug membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
• Immunogen: Amino acid sequence near the 4th C-terminal intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected is not present in A1, A2b and A3 receptors.
• Cross Reactivity: Bovine, Human, Porcine, Rat
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-ADORA2A antibody

The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b, and A3. The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular and the C-termini cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body.

Product Categories/Family for anti-ADORA2A antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 5921992
• NCBI GeneID: 135
• NCBI Accession #: NP_000666.2
• NCBI GenBank Nucleotide #: NM_000675.4
• UniProt Accession #: P29274; B2R7E0; Q93003
• NCBI Official Full Name: adenosine receptor A2a
• NCBI Official Synonym Full Names: adenosine A2a receptor
• NCBI Official Symbol: ADORA2A
• NCBI Official Synonym Symbols: RDC8; hA2aR; ADORA2
• NCBI Protein Information: adenosine receptor A2a; OTTHUMP00000198454; adenosine A2 receptor; adenosine receptor subtype A2a
• UniProt Protein Name: Adenosine receptor A2a
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA2A
• UniProt Synonym Gene Names: ADORA2
• UniProt Entry Name: AA2AR_HUMAN

NCBI Description

This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. [provided by RefSeq]

Uniprot Description

ADORA2A: one of several receptor subtypes for adenosine. A G-protein coupled receptor. Activation is mediated by G proteins which activate adenylyl cyclase. Abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: identical protein binding; protein binding; enzyme binding; adenosine receptor activity, G-protein coupled

Biological Process: central nervous system development; nerve growth factor receptor signaling pathway; blood circulation; apoptosis; adenylate cyclase activation; phagocytosis; G-protein signaling, coupled to cAMP nucleotide second messenger; cell-cell signaling; sensory perception; cellular defense response; cAMP biosynthetic process; blood coagulation; inflammatory response; adenosine receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway