Rabbit ADD3 Polyclonal Antibody | anti-ADD3 antibody

Rabbit Polyclonal (IgG) to Human ADD3

Cat. Num. AAA248710

• Gene Names: ADD3; ADDL
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ADD3; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human ADD3; Anti-ADD3 Antibody (aa431-480) IHC-plus; ADDL; Adducin-like protein 70; Adducin 3 (gamma); Adducin gamma subunit; Gamma-adducin; Human ADD3; anti-ADD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ADD3 Antibody detects endogenous levels of total ADD3 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 706

• Applicable Applications for anti-ADD3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human ADD3 (aa431-480).
• Immunogen: ADD3 antibody was raised against synthetic peptide from human ADD3 (aa431-480).
• Antigen Modification: aa431-480
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-ADD3 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from HepG2/293 cells, using ADD3 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 9943848
• NCBI GeneID: 120
• NCBI Accession #: NP_058432.1
• NCBI GenBank Nucleotide #: NM_016824.3
• UniProt Accession #: Q9UEY8; O43243; Q5VU09; Q92773; Q9UEY7; D3DRA8
• Molecular Weight: 75,671 Da
• NCBI Official Full Name: gamma-adducin isoform a
• NCBI Official Synonym Full Names: adducin 3 (gamma)
• NCBI Official Symbol: ADD3
• NCBI Official Synonym Symbols: ADDL
• NCBI Protein Information: gamma-adducin; adducin-like protein 70
• UniProt Protein Name: Gamma-adducin
• Protein Family: Gamma-adducin
• UniProt Gene Name: ADD3
• UniProt Synonym Gene Names: ADDL
• UniProt Entry Name: ADDG_HUMAN

NCBI Description

Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq, Jul 2008]

Uniprot Description

ADD3: is a heteromeric protein composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducins alpha and gamma are ubiquitously expressed, while the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions of alpha and beta have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: nucleoplasm; condensed nuclear chromosome; cytoskeleton; membrane; cytoplasm; plasma membrane; intercellular junction; cell cortex

Molecular Function: calmodulin binding; structural constituent of cytoskeleton; actin binding