Rabbit anti-Human ADCY8 Polyclonal Antibody | anti-ADCY8 antibody

ADCY8, ID (Ca(2+)/Calmodulin-activated Adenylyl Cyclase, Adenylyl Cyclase 8, ATP Pyrophosphate-lyase 8, Adenylate Cyclase Type VIII, Adenylate Cyclase Type 8) (PE)

Cat. Num. AAA6461586

• Gene Names: ADCY8; AC8; ADCY3; HBAC1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ADCY8; Polyclonal Antibody; ID (Ca(2+)/Calmodulin-activated Adenylyl Cyclase; Adenylyl Cyclase 8; ATP Pyrophosphate-lyase 8; Adenylate Cyclase Type VIII; Adenylate Cyclase Type 8) (PE); anti-ADCY8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ADCY8.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ADCY8 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ADCY8 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ADCY8 Antibody (Center) in HL-60 cell line lysates (35ug/lane). ADCY8 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded mouse brain tissue reacted with ADCY8 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ADCY8 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ADCY8 antibody

Antibodies; Enzymes; Cyclase

References

1. Martin, A.C., et.al., Mol. Pharmacol. 75(4), 830-842 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4557257
• NCBI GeneID: 114
• NCBI Accession #: NP_001106.1
• NCBI GenBank Nucleotide #: NM_001115.2
• UniProt Accession #: P40145
• Molecular Weight: 140,122 Da
• NCBI Official Full Name: adenylate cyclase type 8
• NCBI Official Synonym Full Names: adenylate cyclase 8 (brain)
• NCBI Official Symbol: ADCY8
• NCBI Official Synonym Symbols: AC8; ADCY3; HBAC1
• NCBI Protein Information: adenylate cyclase type 8; adenylyl cyclase 8; ATP pyrophosphate-lyase 8; adenylyl cyclase-8, brain; adenylate cyclase type VIII; ca(2+)/calmodulin-activated adenylyl cyclase
• UniProt Protein Name: Adenylate cyclase type 8
• Protein Family: Adenylate cyclase
• UniProt Gene Name: ADCY8
• UniProt Synonym Gene Names: AC8
• UniProt Entry Name: ADCY8_HUMAN

NCBI Description

Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. The enzymatic activity is under the control of several hormones, and different polypeptides participate in the transduction of the signal from the receptor to the catalytic moiety. Stimulatory or inhibitory receptors (Rs and Ri) interact with G proteins (Gs and Gi) that exhibit GTPase activity and they modulate the activity of the catalytic subunit of the adenylyl cyclase [provided by RefSeq, Jul 2008]

Uniprot Description

ADCY8: This is a membrane-bound, calcium-stimulable adenylyl cyclase. May be involved in learning, in memory and in drug dependence. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Protein type: Transporter; Nucleotide Metabolism - purine; Lyase; Membrane protein, multi-pass; EC 4.6.1.1; Membrane protein, integral; Transporter, aquaporin family; Adenylyl cyclase

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: membrane; plasma membrane; integral to membrane

Molecular Function: metal ion binding; calcium- and calmodulin-responsive adenylate cyclase activity; ATP binding; adenylate cyclase activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; signal transduction; G-protein signaling, adenylate cyclase activating pathway; synaptic transmission; learning and/or memory; long-term memory; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; transmembrane transport