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Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate,A549 whole cell lysate,HepG2 whole cell lysateAll lanes: ADCK4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61,56 KDaObserved band size: 61,56 KDa)

Rabbit anti-Human ADCK4 Polyclonal Antibody | anti-ADCK4 antibody

ADCK4 Antibody

Gene Names
COQ8B; ADCK4; NPHS9
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
ADCK4; Polyclonal Antibody; ADCK4 Antibody; AarF domain-containing protein kinase 4; anti-ADCK4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
503
Applicable Applications for anti-ADCK4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
Immunogen
Recombinant Human AarF domain-containing protein kinase 4 protein (10-137AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-130114 / sc-130114
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate,A549 whole cell lysate,HepG2 whole cell lysateAll lanes: ADCK4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61,56 KDaObserved band size: 61,56 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate,A549 whole cell lysate,HepG2 whole cell lysateAll lanes: ADCK4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 61,56 KDaObserved band size: 61,56 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7050823 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7050823 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human melanoma cancer using MBS7050823 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human melanoma cancer using MBS7050823 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of A549 cells using MBS7050823 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of A549 cells using MBS7050823 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-ADCK4 antibody
May play a role in CoQ10 (COQ10A and/or COQ10B) biosynthesis, which is required for podocyte migration.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55,853 Da
NCBI Official Full Name
atypical kinase COQ8B, mitochondrial isoform b
NCBI Official Synonym Full Names
coenzyme Q8B
NCBI Official Symbol
COQ8B
NCBI Official Synonym Symbols
ADCK4; NPHS9
NCBI Protein Information
atypical kinase COQ8B, mitochondrial
UniProt Protein Name
Atypical kinase COQ8B, mitochondrial
UniProt Gene Name
COQ8B
UniProt Entry Name
COQ8B_HUMAN

NCBI Description

This gene encodes a protein with two copies of a domain found in protein kinases. The encoded protein has a complete protein kinase catalytic domain, and a truncated domain that contains only the active and binding sites of the protein kinase domain, however, it is not known whether the protein has any kinase activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

ADCK4: The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr). Belongs to the protein kinase superfamily. ADCK protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: ABC1 family; ABC1-A subfamily; ATYPICAL group; EC 2.7.-.-; EC 2.7.11.-; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical

Chromosomal Location of Human Ortholog: 19q13.2

Molecular Function: protein kinase activity

Biological Process: cerebellar Purkinje cell layer morphogenesis; protein amino acid phosphorylation; ubiquinone biosynthetic process

Disease: Nephrotic Syndrome, Type 9

Research Articles on ADCK4

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Product Notes

The ADCK4 coq8b (Catalog #AAA7050823) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADCK4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADCK4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1: 500-1: 5000 IHC: 1: 20-1: 200 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the ADCK4 coq8b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADCK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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