Rabbit anti-Human, Mouse ADAP Polyclonal Antibody | anti-FYB antibody

ADAP Antibody

Cat. Num. AAA150722

• Gene Names: FYB; ADAP; PRO0823; SLAP130; SLAP-130
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: ADAP Antibody is affinity chromatography purified via peptide column.
• Synonyms: ADAP; Polyclonal Antibody; ADAP Antibody; PRO0823; SLAP-130; SLAP130; FYN-binding protein; Adhesion and degranulation promoting adaptor protein; FYN binding protein (FYB-120/130); anti-FYB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ADAP Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 829

• Applicable Applications for anti-FYB antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ADAP antibody can be used for detection of ADAP by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ADAP antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human ADAP.
• Buffer: ADAP Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ADAP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 μg/mL.)

Related Product Information for anti-FYB antibody

ADAP Antibody: The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-κB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 42476118
• NCBI GeneID: 2533
• NCBI Accession #: NP_001456
• NCBI GenBank Nucleotide #: NM_001465.4
• UniProt Accession #: O15117; O00359; Q9NZI9; A8K2Y8; B4DLN2; E9PBV9
• Molecular Weight: 91,651 Da
• NCBI Official Full Name: FYN-binding protein isoform 1
• NCBI Official Synonym Full Names: FYN binding protein
• NCBI Official Symbol: FYB
• NCBI Official Synonym Symbols: ADAP; PRO0823; SLAP130; SLAP-130
• NCBI Protein Information: FYN-binding protein; p120/p130; FYB-120/130; FYN-T-binding protein; SLP-76-associated phosphoprotein; adhesion and degranulation-promoting adaptor protein
• UniProt Protein Name: FYN-binding protein
• Protein Family: Adaptin
• UniProt Gene Name: FYB
• UniProt Synonym Gene Names: SLAP130; ADAP; p120/p130
• UniProt Entry Name: FYB_HUMAN

NCBI Description

The protein encoded by this gene is an adapter for the FYN protein and LCP2 signaling cascades in T-cells. The encoded protein is involved in platelet activation and controls the expression of interleukin-2. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

SLAP-130: an adapter protein for fyn and the SH2-domain-containing leucocyte protein-76 (SLP76) in T cells. T-cell receptor ligation leads to increased tyrosine phosphorylation of SLAP-130. Two alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: cytosol; nucleus; actin cytoskeleton

Molecular Function: protein binding; protein complex binding; receptor binding

Biological Process: immune response; NLS-bearing substrate import into nucleus; signal transduction; T cell receptor signaling pathway; protein amino acid phosphorylation