Rabbit anti-Human ADAM12 Polyclonal Antibody | anti-ADAM12 antibody

Anti-ADAM12 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104288

• Gene Names: ADAM12; MCMP; MLTN; MLTNA; MCMPMltna
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: ADAM12; Polyclonal Antibody; Anti-ADAM12 Antibody; Rabbit Polyclonal; ADAM12/MLTN Antibody; Rabbit PAb; Antigen Affinity Purified; ADAM metallopeptidase domain 12; Anti-ADAM12-OT1 Antibody; Anti-CAR10 Antibody; Anti-MCMP Antibody; Anti-MCMPMltna Antibody; Anti-MLTN Antibody; Anti-MLTNA Antibody; anti-ADAM12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ADAM12/MLTN
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ADAM12 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human ADAM12 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ADAM12/MLTN extracellular domain (rh ADAM12). ADAM12 specific IgG was purified by human ADAM12 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-ADAM12 antibody

The ADAMs (a disintegrin and metalloprotease) comprise a family of multidomain proteins with metalloprotease, cell adhesion, and signaling activities. Human ADAM12, which is implicated in diseases such as cancer, is expressed in two splice forms, the transmembrane ADAM12-L and the shorter and soluble ADAM12-S. ADAM12, also known as and Meltrin alpha, is a member of the ADAM protein family, which contains one disintegrin domain, one EGF-like domain and one peptidase M12B domain. ADAM12 is synthesized as a zymogen with the prodomain keeping the metalloprotease inactive through a cysteine-switch mechanism. Maturation and activation of the protease involves the cleavage of the prodomain in the trans-Golgi or possibly at the cell surface by a furin-peptidase. It is a membrane-anchored metalloprotease, which has been implicated in activation-inactivation of growth factors that play an important role in wound healing, including heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) and IGF binding proteins. ADAM12 may also regulate cell-cell and cell-extracellular matrix contacts through interactions with cell surface receptors-integrins and syndecans-potentially influencing the actin cytoskeleton. Moreover, ADAM12 interacts with several cytoplasmic signaling and adaptor molecules through its intracellular domain, thereby directly transmitting signals to or from the cell interior. These ADAM12-mediated cellular effects appear to be critical events in both biological and pathological processes. In addition to protease activity, ADAM12 possesses cell binding and cell signaling properties. In many studies, ADAM12 overexpression has been correlated with disease, and ADAM12 has been shown to promote tumor growth and progression in cancer. On the other hand, protective effects of ADAM12 in disease have also been reported.

Product Categories/Family for anti-ADAM12 antibody

Notch Signaling

References

Wewer UM, et al. (2006) ADAM12 is a four-leafed clover: the excised prodomain remains bound to the mature enzyme. J Biol Chem. 281(14): 9418-22.Kveiborg M, et al. (2008) Cellular roles of ADAM12 in health and disease. Int J Biochem Cell Biol. 40(9): 1685-702.Harsha A, et al. (2008) ADAM12: a potential target for the treatment of chronic wounds. J Mol Med. 86(8): 961-9.Jacobsen J, et al. (2009) Targeting ADAM12 in human disease: head, body or tail? Curr Pharm Des. 15(20): 2300-10.Baertling F, et al. (2010) ADAM12 is expressed by astrocytes during experimental demyelination. Brain Res. 1326: 1-14.

NCBI and Uniprot Product Information

• NCBI GI #: 73747885
• NCBI GeneID: 8038
• NCBI Accession #: NP_003465.3
• NCBI GenBank Nucleotide #: NM_003474.4
• UniProt Accession #: O43184; O60470; Q5JRP0; Q5JRP1; Q6P9E3; Q6UWB0
• Molecular Weight: 99,542 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 12 isoform 1 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 12
• NCBI Official Symbol: ADAM12
• NCBI Official Synonym Symbols: MCMP; MLTN; MLTNA; MCMPMltna
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 12; meltrin-alpha
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 12
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM12
• UniProt Synonym Gene Names: MLTN; ADAM 12
• UniProt Entry Name: ADA12_HUMAN

Uniprot Description

ADAM12: contains a disintegrin and metalloprotease (ADAM) domain 12, which is a member of the ADAM protein family. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion, and the differentiation of macrophage-derived giant cells (MGC) and osteoclasts. 2 alternative splicing isoforms have been described: a shorter secreted form and a longer membrane-bound form. The shorter form stimulates myogenesis.

Protein type: Protease; Membrane protein, integral; EC 3.4.24.-; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: nucleoplasm; mitochondrion; integral to membrane; plasma membrane; extracellular region

Molecular Function: protein binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; SH3 domain binding

Biological Process: epidermal growth factor receptor signaling pathway; proteolysis; cell adhesion; myoblast fusion