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Western Blot (WB) (Anti-CD156c rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:84 kDaObserved band size:65 kDa(We are unsure as to the identity of these extra bands.))

Rabbit ADAM10 Polyclonal Antibody | anti-ADAM10 antibody

Anti-ADAM10 Antibody, Rabbit Polyclonal

Gene Names
ADAM10; kuz; AD10; MADM; CD156c; HsT18717
Reactivity
Human
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
ADAM10; Polyclonal Antibody; Anti-ADAM10 Antibody; Rabbit Polyclonal; ADAM10/CD156c Antibody; Rabbit PAb; Antigen Affinity Purified; ADAM metallopeptidase domain 10; Anti-AD10 Antibody; Anti-AD18 Antibody; Anti-CD156c Antibody; Anti-HsT18717 Antibody; Anti-kuz Antibody; Anti-MADM Antibody; Anti-RAK Antibody; anti-ADAM10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ADAM10
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ADAM10 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to the C-terminus of the human CD156c
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human CD156c, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CD156c rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:84 kDaObserved band size:65 kDa(We are unsure as to the identity of these extra bands.))

Western Blot (WB) (Anti-CD156c rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:84 kDaObserved band size:65 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-ADAM10 antibody
A disintegrin and metalloproteinase 10 (ADAM10) is a ubiquitous transmembrane protein that functions as a "molecular scissor" to cleave the extracellular regions from its transmembrane target proteins. ADAM10 is well characterized as the ligand-dependent activator of Notch proteins, which control cell fate decisions. The transmembrane protein, ADAM10 (a disintegrin and metalloprotease 10), has key physiologic functions-for example, during embryonic development and in the brain. During transit through the secretory pathway, immature ADAM10 (proADAM10) is converted into its proteolytically active, mature form (mADAM10). Increasing or decreasing the abundance and/or activity of mADAM10 is considered to be a therapeutic approach for the treatment of such diseases as Alzheimer's disease and cancer. Yet biochemical detection and characterization of mADAM10 have been difficult.
Product Categories/Family for anti-ADAM10 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
102
UniProt Accession #
Molecular Weight
84,142 Da
NCBI Official Full Name
ADAM10
NCBI Official Synonym Full Names
ADAM metallopeptidase domain 10
NCBI Official Symbol
ADAM10
NCBI Official Synonym Symbols
kuz; AD10; MADM; CD156c; HsT18717
NCBI Protein Information
disintegrin and metalloproteinase domain-containing protein 10; CDw156; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; a disintegrin and metalloproteinase domain 10
UniProt Protein Name
Disintegrin and metalloproteinase domain-containing protein 10
UniProt Gene Name
ADAM10
UniProt Synonym Gene Names
KUZ; MADM; ADAM 10
UniProt Entry Name
ADA10_HUMAN

NCBI Description

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

Protein type: Cell adhesion; Vesicle; Motility/polarity/chemotaxis; Protease; Membrane protein, integral; EC 3.4.24.81; Cell surface

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: Golgi apparatus; cell surface; focal adhesion; membrane; intracellular membrane-bound organelle; cytoplasm; postsynaptic density; plasma membrane; integral to membrane; Golgi-associated vesicle; nucleus

Molecular Function: integrin binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; endopeptidase activity; protein kinase binding; SH3 domain binding; receptor binding

Biological Process: integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; axon guidance; extracellular matrix organization and biogenesis; Notch signaling pathway; in utero embryonic development; constitutive protein ectodomain proteolysis; membrane protein ectodomain proteolysis; Notch receptor processing; negative regulation of cell adhesion; protein amino acid phosphorylation; positive regulation of cell growth; extracellular matrix disassembly; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; monocyte activation; cell-cell signaling; positive regulation of cell proliferation; ephrin receptor signaling pathway; positive regulation of cell migration

Disease: Reticulate Acropigmentation Of Kitamura; Alzheimer Disease 18

Research Articles on ADAM10

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Product Notes

The ADAM10 adam10 (Catalog #AAA8100323) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ADAM10 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Rat (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's ADAM10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the ADAM10 adam10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAM10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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