Rabbit anti-Human, Mouse ACVRL1 Polyclonal Antibody | anti-ACVRL1 antibody

ACVRL1, NT (ACVRL1, ACVRLK1, ALK1, Serine/threonine-protein kinase receptor R3, Activin receptor-like kinase 1, TGF-B superfamily receptor type I) (PE)

Cat. Num. AAA6271452

• Gene Names: ACVRL1; HHT; ALK1; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: ACVRL1; Polyclonal Antibody; NT (ACVRL1; ACVRLK1; ALK1; Serine/threonine-protein kinase receptor R3; Activin receptor-like kinase 1; TGF-B superfamily receptor type I) (PE); TGF-B superfamily receptor type I; anti-ACVRL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACVRL1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ACVRL1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 38-68 in the N-terminal region of human ACVRL1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot of mouse heart tissue lysate usingMBS641713.)

Western Blot (WB)

(Western Blot analysis of ACVRL1 (arrow) of 293 cell lysates (2ug/lane) usingMBS641713. Lane 1: nontransfected Lane 2: transiently transfected with the ACVRL1 gene Origene Technologies).)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed and paraffin-embedded human hepatocarcinoma. tissue rusingMBS641713, followed by peroxidase-conjugated to the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HepG2 cells using MBS641713 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ACVRL1 antibody

Antibodies; Protein Kinases

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). 2. Berg, J.N., et al., Am. J. Hum. Genet. 61(1):60-67 (1997). 3. Johnson, D.W., et al., Nat. Genet. 13(2):189-195 (1996). 4. ten Dijke, P., et al., Oncogene 8(10):2879-2887 (1993). 5. Attisano, L., et al., Cell 75(4):671-680 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 116734712
• NCBI GeneID: 94
• NCBI Accession #: NP_000011.2
• NCBI GenBank Nucleotide #: NM_000020.2
• UniProt Accession #: P37023; A6NGA8
• Molecular Weight: 56,124 Da
• NCBI Official Full Name: serine/threonine-protein kinase receptor R3
• NCBI Official Synonym Full Names: activin A receptor type II-like 1
• NCBI Official Symbol: ACVRL1
• NCBI Official Synonym Symbols: HHT; ALK1; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1
• NCBI Protein Information: serine/threonine-protein kinase receptor R3; TGF-B superfamily receptor type I; activin A receptor, type II-like kinase 1
• UniProt Protein Name: Serine/threonine-protein kinase receptor R3
• Protein Family: Serine/threonine-protein kinase receptor
• UniProt Gene Name: ACVRL1
• UniProt Synonym Gene Names: ACVRLK1; ALK1; SKR3; ALK-1; TSR-I
• UniProt Entry Name: ACVL1_HUMAN

NCBI Description

This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine/threonine kinases. Mutations in this gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

ALK1: a kinase of the STKR family. A type I cell-surface receptor for the TGF-beta superfamily of ligands including Activin A. Highly expressed in human placenta and lung. Deficiency causes hemorrhagic telangiectasia type 2 also known as Rendu-Osler-Weber syndrome 2.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, Ser/Thr (receptor); EC 2.7.11.30; Protein kinase, TKL; TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cell surface; cell soma; integral to plasma membrane; dendrite

Molecular Function: transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; transforming growth factor beta binding; transforming growth factor beta receptor activity, type I; metal ion binding; activin binding; SMAD binding; protein kinase binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: blood circulation; protein heterooligomerization; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; negative regulation of cell adhesion; signal transduction; regulation of blood vessel endothelial cell migration; protein amino acid phosphorylation; BMP signaling pathway; negative regulation of cell proliferation; positive regulation of endothelial cell differentiation; regulation of transcription, DNA-dependent; negative regulation of endothelial cell differentiation; transforming growth factor beta receptor signaling pathway; regulation of blood pressure; lymphangiogenesis; angiogenesis; negative regulation of cell migration; positive regulation of BMP signaling pathway; regulation of DNA replication; regulation of endothelial cell proliferation; in utero embryonic development; negative regulation of focal adhesion formation; negative regulation of blood vessel endothelial cell migration; blood vessel maturation; blood vessel endothelial cell proliferation during sprouting angiogenesis; positive regulation of angiogenesis; positive regulation of chondrocyte differentiation; negative regulation of endothelial cell proliferation; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; negative regulation of cell growth; wound healing, spreading of epidermal cells

Disease: Telangiectasia, Hereditary Hemorrhagic, Type 2