Rabbit anti-Human, Mouse ACVR1 Polyclonal Antibody | anti-ACVR1 antibody

ACVR1, NT (Activin Receptor Type 1, Activin Receptor Type I, ACTR-I, Serine/Threonine-protein Kinase Receptor R1, SKR1, Activin Receptor-like Kinase 2, ALK-2, TGF-B Superfamily Receptor Type I, TSR-I, ACVRLK2) (MaxLight 750)

Cat. Num. AAA6461387

• Gene Names: ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation
• Synonyms: ACVR1; Polyclonal Antibody; NT (Activin Receptor Type 1; Activin Receptor Type I; ACTR-I; Serine/Threonine-protein Kinase Receptor R1; SKR1; Activin Receptor-like Kinase 2; ALK-2; TGF-B Superfamily Receptor Type I; TSR-I; ACVRLK2) (MaxLight 750); anti-ACVR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ACVR1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Ammonium Sulfate Precipitation
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-ACVR1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa20-50 in the N-terminal region of human ACVR1, UniProt Accession #NP_001096; Q04771
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ACVR1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the ACVR1 antibody detected the ACVR1 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of anti-ACVR1 Pab in mouse brain tissue lysate.ACVR1(arrow) was detected using the purified Pab.)

Product Categories/Family for anti-ACVR1 antibody

Antibodies; Activin Receptors

References

1. Casagrandi, D., et al., Mol. Hum. Reprod. 9(4):199-203 (2003). 2. Welt, C.K., Curr Opin Obstet Gynecol 14(3):317-323 (2002). 3. Schneider-Kolsky, M.E., et al., Placenta 23(4):294-302 (2002). 4. Chapman, S.C., et al., Mol. Endocrinol. 15(4):668-679 (2001). 5. Schulte, K.M., et al., Horm. Metab. Res. 32(10):390-400 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4501895
• NCBI GeneID: 90
• NCBI Accession #: NP_001096
• NCBI GenBank Nucleotide #: NP_001096.1
• UniProt Accession #: Q04771
• NCBI Official Full Name: activin receptor type-1
• NCBI Official Synonym Full Names: activin A receptor type 1
• NCBI Official Symbol: ACVR1
• NCBI Official Synonym Symbols: FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
• NCBI Protein Information: activin receptor type-1
• UniProt Protein Name: Activin receptor type-1
• Protein Family: Activin receptor
• UniProt Gene Name: ACVR1
• UniProt Synonym Gene Names: ACVRLK2; ACTR-I; ALK-2; SKR1; TSR-I
• UniProt Entry Name: ACVR1_HUMAN

NCBI Description

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive. [provided by RefSeq, Jul 2008]

Uniprot Description

ALK2: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis. Interacts with FKBP1A. Interacts with FCHO1. Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Protein type: Membrane protein, integral; Kinase, protein; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 2q23-q24

Cellular Component: apical part of cell; integral to plasma membrane; activin receptor complex

Molecular Function: protein homodimerization activity; metal ion binding; peptide hormone binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; transforming growth factor beta receptor activity, type I; transforming growth factor beta binding; activin binding; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: pharyngeal system development; in utero embryonic development; positive regulation of transcription, DNA-dependent; peptidyl-threonine phosphorylation; activin receptor signaling pathway; gastrulation with mouth forming second; positive regulation of bone mineralization; protein amino acid phosphorylation; germ cell development; negative regulation of signal transduction; BMP signaling pathway; patterning of blood vessels; positive regulation of osteoblast differentiation; negative regulation of activin receptor signaling pathway; mesoderm formation; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; regulation of skeletal muscle development; determination of left/right symmetry; regulation of ossification; neural crest cell migration; acute inflammatory response; urogenital system development; G1/S transition of mitotic cell cycle

Disease: Fibrodysplasia Ossificans Progressiva