Rabbit anti-Human ACSL3 Polyclonal Antibody | anti-ACSL3 antibody

ACSL3, CT (ACSL3, ACS3, FACL3, LACS3, Long-chain-fatty-acid-CoA ligase 3, Long-chain acyl-CoA synthetase 3) (PE)

Cat. Num. AAA6271111

• Gene Names: ACSL3; ACS3; FACL3; LACS3; LACS 3; PRO2194
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ACSL3; Polyclonal Antibody; CT (ACSL3; ACS3; FACL3; LACS3; Long-chain-fatty-acid-CoA ligase 3; Long-chain acyl-CoA synthetase 3) (PE); Long-chain-fatty-acid--CoA ligase 3; Long-chain acyl-CoA synthetase 3; anti-ACSL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 720

• Applicable Applications for anti-ACSL3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ACSL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-556 amino acids from the C-terminal region of human ACSL3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ACSL3 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ACSL3 antibody detected the ACSL3 protein (arrow).)

Related Product Information for anti-ACSL3 antibody

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-ACSL3 antibody

Antibodies; Enzymes; Synthase

References

Weedon-Fekjaer, M.S., et al. J. Lipid Res. 51(7):1886-1896(2010). Cao, A., et al. J. Biol. Chem. 285(22):16664-16674(2010). Perera, F., et al. PLoS ONE 4 (2), E4488 (2009): Yao, H., et al. J. Biol. Chem. 283(2):849-854(2008). Jia, Z., et al. J. Mol. Neurosci. 33(1):25-31(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 42794754
• NCBI GeneID: 2181
• NCBI Accession #: NP_976251.1; NP_004448.2
• NCBI GenBank Nucleotide #: NP_976251.1
• UniProt Accession #: O95573
• NCBI Official Full Name: long-chain-fatty-acid--CoA ligase 3
• NCBI Official Synonym Full Names: acyl-CoA synthetase long chain family member 3
• NCBI Official Symbol: ACSL3
• NCBI Official Synonym Symbols: ACS3; FACL3; LACS3; LACS 3; PRO2194
• NCBI Protein Information: long-chain-fatty-acid--CoA ligase 3
• UniProt Protein Name: Long-chain-fatty-acid--CoA ligase 3
• Protein Family: Long-chain-fatty-acid--CoA ligase
• UniProt Gene Name: ACSL3
• UniProt Synonym Gene Names: ACS3; FACL3; LACS3; LACS 3

NCBI Description

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACSL3: Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). Belongs to the ATP-dependent AMP-binding enzyme family.

Protein type: EC 6.2.1.3; Ligase; Lipid Metabolism - fatty acid; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q36.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi apparatus; lipid particle; membrane; perinuclear region of cytoplasm

Molecular Function: long-chain-fatty-acid-CoA ligase activity; protein domain specific binding; protein kinase binding

Biological Process: positive regulation of Golgi to plasma membrane protein transport; positive regulation of secretion