Goat ACPP Polyclonal Antibody | anti-ACPP antibody

Goat anti-ACPP Antibody

Cat. Num. AAA422261

• Gene Names: ACPP; ACP3; 5'-NT; ACP-3
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: ACPP; Polyclonal Antibody; Goat anti-ACPP Antibody; Goat Anti-ACPP / PAP (aa269-282) Antibody; acid phosphatase; prostate; ACP-3; ACP3; PAP; prostatic acid phosphotase; ACPP antibody; prostate antibody; ACP-3 antibody; ACP3 antibody; PAP antibody; prostatic acid phosphotase antibody; anti-ACPP antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: YGIHKQKEKSRLQ
• Sequence Length: 386

• Applicable Applications for anti-ACPP antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Immunohistochemistry: In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5ug/ml.; Western Blot: Approx 50kDa band observed in Human Prostate lysates (calculated MW of 44.6kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al,,Clin Chem.1986 Oct;32(10): 1832-5. PMID: 3530530). Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence C-YGIHKQKEKSRLQ, from the internal region of the protein sequence according to NP_001090.2.
• Epitope: Internal region
• Note: This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

((3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 6382064
• NCBI GeneID: 55
• NCBI Accession #: NP_001090.2
• NCBI GenBank Nucleotide #: NM_001099.4
• Molecular Weight: 40,443 Da
• NCBI Official Full Name: prostatic acid phosphatase isoform PAP
• NCBI Official Synonym Full Names: acid phosphatase, prostate
• NCBI Official Symbol: ACPP
• NCBI Official Synonym Symbols: ACP3; 5'-NT; ACP-3
• NCBI Protein Information: prostatic acid phosphatase
• UniProt Protein Name: Prostatic acid phosphatase
• Protein Family: Acyl carrier protein
• UniProt Gene Name: ACPP
• UniProt Synonym Gene Names: PAP; 5'-NT; TMPase
• UniProt Entry Name: PPAP_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. An alternatively spliced transcript variant encoding a longer isoform has been found for this gene. This isoform contains a transmembrane domain and is localized in the plasma membrane-endosomal-lysosomal pathway. [provided by RefSeq, Sep 2008]

Uniprot Description

A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.